Oral Administration of the Endocannabinoid Anandamide during Lactation: Effects on Hypothalamic Cannabinoid Type 1 Receptor and Food Intake in Adult Mice
| Autor: | Valeska Castillo, Carolina Aguirre, Miguel N. Llanos |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Article Subject Endocrinology Diabetes and Metabolism medicine.medical_treatment Biology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Insulin resistance Western blot Oral administration Internal medicine Lactation medicine Receptor lcsh:RC620-627 Nutrition and Dietetics medicine.diagnostic_test Anandamide medicine.disease Endocannabinoid system lcsh:Nutritional diseases. Deficiency diseases 030104 developmental biology Endocrinology medicine.anatomical_structure chemistry lipids (amino acids peptides and proteins) Cannabinoid 030217 neurology & neurosurgery Research Article Food Science |
| Zdroj: | Journal of Nutrition and Metabolism Journal of Nutrition and Metabolism, Vol 2017 (2017) |
| ISSN: | 2090-0732 2090-0724 |
| DOI: | 10.1155/2017/2945010 |
| Popis: | We have previously shown that administration of the endocannabinoid anandamide (AEA) during lactation leads to overweight, increased body fat accumulation, and insulin resistance in adult mice. This study was designed to elucidate if these effects are due to increased food intake, stimulated by an augmented abundance and binding ability of the hypothalamic cannabinoid type 1 receptor (CB1R). With this aim, male mice pups were treated with a daily oral dose of AEA during lactation. Adult mice were also treated with a single oral dose of AEA, to evaluate acute food intake during 4 h. At 21 and 160 days, CB1R protein abundance was calculated by western blot analysis. Capacity of hypothalamic membranes to specifically bind the radioligand3[H]-CP55.940 was also measured. Western blots showed a 72% increase in CB1R abundance in AEA-treated 21-day-old mice, without differences in adult mice. Additionally, specific binding of3[H]-CP55.940 to hypothalamic membranes from adult mice was significantly lower in those mice treated with AEA during lactation. Moreover, AEA did not stimulate acute food intake in both, AEA-treated and control mice. Results suggest that metabolic alterations found in adult mice because of AEA treatment during lactation are not associated with hypothalamic CB1R. |
| Databáze: | OpenAIRE |
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