Effect of ultrasonic streaming on intra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment
Autor: | Amanda Garcia Alves Maliza, Raquel Zanin Midena, Marcela P. C. Arias, Márcia Sirlene Zardin Graeff, Flaviana Bombarda de Andrade, Marco Antonio Hungaro Duarte |
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Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
medicine.medical_specialty Materials science Time Factors Ultrasonic Therapy 030106 microbiology Colony Count Microbial Dentistry AGENTES ANTIMICROBIANOS Enterococcus faecalis Endodontics Calcium Hydroxide 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Ultrasound Dentin medicine Animals General Dentistry Chromatography Calcium hydroxide Microbial Viability Microscopy Confocal biology Root Canal Irrigants business.industry Reproducibility of Results 030206 dentistry Penetration (firestop) Original Articles biology.organism_classification Root Canal Therapy lcsh:RK1-715 medicine.anatomical_structure Dentinal Tubule chemistry lcsh:Dentistry Anti-Infective Agents Local Ultrasonic sensor Cattle Dental Pulp Cavity business |
Zdroj: | Journal of Applied Oral Science Journal of Applied Oral Science, Volume: 24, Issue: 6, Pages: 575-581, Published: DEC 2016 Journal of Applied Oral Science v.24 n.6 2016 Journal of applied oral science Universidade de São Paulo (USP) instacron:USP Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Journal of Applied Oral Science, Vol 24, Iss 6, Pp 575-581 |
ISSN: | 1678-7765 1678-7757 |
Popis: | Objective The antimicrobial effect of ultrasonic agitation of calcium hydroxide (CH) pastes in infected bovine dentin and their penetrability were evaluated using confocal laser scanning microscopy (CLSM) and microbiological culture. Material and Methods Fifty-two bovine teeth were infected with Enterococcus faecalis using a new contamination protocol; then they received CH paste and were divided into groups with or without ultrasound. Ultrasonic agitation was conducted for 1 min with a plain point insert. After 15 d, the CLSM analyzed the viable and dead bacteria with Live and Dead assay. The dentinal wall debris was collected by burs, and the colony forming units (CFU/mL) were counted. The penetrability of the paste inside dentinal tubules was tested using the B-rodamine dye. Results The calcium hydroxide paste showed better results with the use of ultrasonic agitation (p |
Databáze: | OpenAIRE |
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