Effect of ultrasonic streaming on intra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment

Autor: Amanda Garcia Alves Maliza, Raquel Zanin Midena, Marcela P. C. Arias, Márcia Sirlene Zardin Graeff, Flaviana Bombarda de Andrade, Marco Antonio Hungaro Duarte
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: Journal of Applied Oral Science
Journal of Applied Oral Science, Volume: 24, Issue: 6, Pages: 575-581, Published: DEC 2016
Journal of Applied Oral Science v.24 n.6 2016
Journal of applied oral science
Universidade de São Paulo (USP)
instacron:USP
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Journal of Applied Oral Science, Vol 24, Iss 6, Pp 575-581
ISSN: 1678-7765
1678-7757
Popis: Objective The antimicrobial effect of ultrasonic agitation of calcium hydroxide (CH) pastes in infected bovine dentin and their penetrability were evaluated using confocal laser scanning microscopy (CLSM) and microbiological culture. Material and Methods Fifty-two bovine teeth were infected with Enterococcus faecalis using a new contamination protocol; then they received CH paste and were divided into groups with or without ultrasound. Ultrasonic agitation was conducted for 1 min with a plain point insert. After 15 d, the CLSM analyzed the viable and dead bacteria with Live and Dead assay. The dentinal wall debris was collected by burs, and the colony forming units (CFU/mL) were counted. The penetrability of the paste inside dentinal tubules was tested using the B-rodamine dye. Results The calcium hydroxide paste showed better results with the use of ultrasonic agitation (p
Databáze: OpenAIRE