CD437 induces apoptosis in ovarian adenocarcinoma cells via ER stress signaling
Autor: | Yoshiko Hoshikawa, Kazue Gonda, Kei Terabayashi, Naoki Terakawa, An Afida Ashla, Hiroaki Itamochi, Tasuku Harada, Ren Nishio, Hiroyuki Tsuchiya, Saori Matsuoka, Akihiro Kurimasa, Goshi Shiota, Yuka Fujimoto, Kyoko Ishii, Akiko Tomita, Hideharu Okamoto, Yumi Watanabe, Keitaro Yamane, Tomohiko Sakabe, Yoshiaki Matsumi, Kazuko Takubo, Remina Ikeda, Yuji Akechi |
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Rok vydání: | 2007 |
Předmět: |
Ovarian Neoplasms
Gene knockdown Chemistry Endoplasmic reticulum Biophysics Apoptosis Cell Biology CHOP Adenocarcinoma Endoplasmic Reticulum Biochemistry Cell biology Oxidative Stress Retinoids Cell culture Cell Line Tumor Unfolded protein response Cytotoxic T cell Humans Inducer Female Molecular Biology Signal Transduction |
Zdroj: | Biochemical and biophysical research communications. 366(3) |
ISSN: | 1090-2104 |
Popis: | A synthetic retinoid, CD437, has been shown to exert potent anti-tumor activity against various types of cancer cell lines, regardless of their sensitivities to natural retinoids. We herein demonstrate that CD437 induces endoplasmic reticulum (ER) stress, including the up-regulation of CHOP, BIP and GADD34 mRNA through ER stress transducer (PERK and IRE1α) activation in an ovarian adenocarcinoma cell line, SKOV3. It was also shown that CD437 induced the CHOP and GADD34 expressions in another four ovarian adenocarcinoma cell lines, indicating that CD437 functions as an ER stress inducer in these cell lines. Moreover, the siRNA-mediated knockdown of inducible CHOP expression prevented the cytotoxic effect of CD437. These results suggest that ER stress plays an important role in the mechanism by which CD437 induces apoptosis in ovarian adenocarcinoma cells. |
Databáze: | OpenAIRE |
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