Protective effects of fenofibrate against acute lung injury induced by intestinal ischemia/reperfusion in mice
| Autor: | Jie Wang, Qian-Kun Zhu, Wei Chen, Yukang Wang, Guizhen He |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Acute Lung Injury Ischemia Lung injury Creatine Gastroenterology Article 03 medical and health sciences chemistry.chemical_compound Mice Fenofibrate Internal medicine medicine.artery medicine Animals Superior mesenteric artery Survival rate Multidisciplinary Lung business.industry Anti-Inflammatory Agents Non-Steroidal medicine.disease Intestines Mice Inbred C57BL Disease Models Animal 030104 developmental biology Endocrinology medicine.anatomical_structure chemistry Reperfusion Injury business Reperfusion injury medicine.drug |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep22044 |
| Popis: | This experiment was conducted to evaluate whether pretreatment with fenofibrate could mitigate acute lung injury (ALI) in a mice model of intestinal ischemia/reperfusion (I/R). Male C57BL/6 mice were randomly assigned into three groups (n = 6): sham, intestinal I/R + vehicle, and intestinal I/R + fenofibrate. Intestinal I/R was achieved by clamping the superior mesenteric artery. Fenofibrate (100 mg/kg) or equal volume of vehicle was injected intraperitoneally 60 minutes before the ischemia. At the end of experiment, measurement of pathohistological score, inflammatory mediators and other markers were performed. In addition, a 24-hour survival experiment was conducted in intestinal I/R mice treated with fenofibrate or vehicle. The chief results were as anticipated. Pathohistological evaluation indicated that fenofibrate ameliorated the local intestine damage and distant lung injury. Pretreatment with fenofibrate significantly decreased inflammatory factors in both the intestine and the lung. Consistently, renal creatine levels and hepatic ALT levels were significantly decreased in the fenofibrate group. Moreover, serum systemic inflammatory response indicators were significantly alleviated in the fenofibrate group. In addition, fenofibrate administration significantly improved the survival rate. Collectively, our data indicated that pretreatment with fenofibrate prior to ischemia attenuated intestinal I/R injury and ALI. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|