Evaluation of genotoxic and antigenotoxic effects of boron by the somatic mutation and recombination test (SMART) onDrosophila

Autor:	Ufuk Sezer, Rabia Sarikaya, Ali Fuat Erciyas, Kamile Erciyas, Muhammed Isa Kara, Sinan Ay
Rok vydání:	2016
Předmět:	inorganic chemicals animal structures Health Toxicology and Mutagenesis chemistry.chemical_element 010501 environmental sciences Biology Toxicology medicine.disease_cause 01 natural sciences 03 medical and health sciences 0302 clinical medicine Germline mutation Phosphoprotein Phosphatases medicine Animals Drosophila Proteins Wings Animal Food science Boron 0105 earth and related environmental sciences Recombination Genetic Pharmacology Larva Chemical Health and Safety Dose-Response Relationship Drug Mutagenicity Tests fungi Public Health Environmental and Occupational Health Antimutagenic Agents Methane sulfonate 030206 dentistry General Medicine biology.organism_classification Drosophila melanogaster chemistry Distilled water Mutation Drosophila Protein Genotoxicity Mutagens
Zdroj:	Drug and Chemical Toxicology. 39:400-406
ISSN:	1525-6014 0148-0545
Popis:	Objective: In this study, different concentrations of boron have been evaluated for genotoxic and antigenotoxic properties by using the somatic mutation and recombination test (SMART) on Drosophila melanogaster. Study Design: The treatment concentrations were chosen to a pretest. Third-instar larvae trans-heterozygous for two genetic markers, multiple wing hair (mwh) and flare (flr3), were treated at different concentrations (0.1, 5, 10, 20, and 40mg/mL) of boron. In addition to investigating antigenotoxic effects, the same boron concentrations were co-administered with 0.1mM Ethyl Methane Sulfonate (EMS). Distilled water was used as a negative control; 0.1mM of EMS was used as a positive control. For the chronic feeding study, small plastic vials were prepared with 1.5g of dry Drosophila Instant Medium and 5mL of the respective test solution. Hundreds of trans-heterozygous larvae were embedded into this medium. Feeding ended with pupation of the surviving larvae. After metamorphosis, all surviving flies were collected and stored in a 70% ethanol solution. Preparation and microscopic analyses of wing were made after the treatment. Then the observed mutations were classified according to size and type of mutation per wing. Results: Results indicated that there is no significant genotoxic effect with all of the boron concentrations. In addition, the antigenotoxic activities of boron against EMS were tested. Results indicated that all boron concentrations (0.1, 5, 10, 20 and 40mg/mL) were able to abolish the genotoxic effects induced by the EMS. Conclusion: It is suggested that the observed effects can be linked to the antioxidant properties of boron. Moreover, these in vivo results will contribute to the antigenotoxicity database of boron.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2f02e9200a9660878feb006c13c31ba8 https://doi.org/10.3109/01480545.2015.1130719 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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