Production of murine monoclonal antibody to fetal hemoglobin

Autor:	Swati Kulkarni, Dipika Mohanty, Vaishali Pujari, Yegneshwar Iyer, Gayatri Shinde, Paritosh Keertikar, Roshan B. Colah, Manisha Patwardhan
Rok vydání:	2003
Předmět:	medicine.drug_class Clinical Biochemistry Cell Blotting Western Monoclonal antibody Sensitivity and Specificity Flow cytometry Mice Fetal hemoglobin medicine Animals Humans Genetics (clinical) Fetal Hemoglobin Immunoassay Mice Inbred BALB C medicine.diagnostic_test biology Biochemistry (medical) Antibodies Monoclonal Hematology Fetal Blood Flow Cytometry Molecular biology medicine.anatomical_structure biology.protein Hemoglobin Antibody Postpartum period
Zdroj:	Hemoglobin. 27(4)
ISSN:	0363-0269
Popis:	Fetal hemoglobin (Hb F) is a major hemoglobin (Hb) component at birth. Hb F levels are markedly elevated in a number of inherited and acquired disorders. Measurement of Hb F levels is usually carried out by alkali denaturation which is not very accurate for low and high values. An accurate estimation of Hb F, and also of F cells, is desired in many hematological disorders like sickle cell disease, in monitoring the efficacy of hydroxyurea (HU) therapy, to assess feto-maternal hemorrhage (FMH) during pregnancy and in the postpartum period. We have raised a murine monoclonal antibody to human Hb F, that accurately measures the number of F cells by flow cytometry. The antibody was found to be potent and specific for F cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2efde389bc9858c77ce4947539ad3181 https://pubmed.ncbi.nlm.nih.gov/14649313 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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