Modular magnetic tweezers for single-molecule characterizations of helicases
| Autor: | Felix E. Kemmerich, Kristina Kasaciunaite, Ralf Seidel |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Scheme (programming language) Magnetic tweezers Optical Tweezers biology business.industry Computer science DNA Helicases Helicase Nanotechnology Modular design General Biochemistry Genetics and Molecular Biology Molecular machine Magnetics 03 medical and health sciences 030104 developmental biology Human–computer interaction biology.protein DNA unwinding business Molecular Biology computer computer.programming_language |
| Zdroj: | Methods. 108:4-13 |
| ISSN: | 1046-2023 |
| DOI: | 10.1016/j.ymeth.2016.07.004 |
| Popis: | Magnetic tweezers provide a versatile toolkit supporting the mechanistic investigation of helicases. In the present article, we show that custom magnetic tweezers setups are straightforward to construct and can easily be extended to provide adaptable platforms, capable of addressing a multitude of enquiries regarding the functions of these fascinating molecular machines. We first address the fundamental components of a basic magnetic tweezers scheme and review some previous results to demonstrate the versatility of this instrument. We then elaborate on several extensions to the basic magnetic tweezers scheme, and demonstrate their applications with data from ongoing research. As our methodological overview illustrates, magnetic tweezers are an extremely useful tool for the characterization of helicases and a custom built instrument can be specifically tailored to suit the experimenter’s needs. |
| Databáze: | OpenAIRE |
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