Plasmodium chabaudi adami: interferon-gamma but not IL-2 is essential for the expression of cell-mediated immunity against blood-stage parasites in mice
| Autor: | Henri C. van der Heyde, Dean D. Manning, Francine Cigel, Barbara J. Pepper, Heather Lieberg, D. M. Yañez, William P. Weidanz, Joan Batchelder, James M. Burns |
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| Rok vydání: | 2003 |
| Předmět: |
Male
T cell Immunology Antibodies Protozoan chemical and pharmacologic phenomena Enzyme-Linked Immunosorbent Assay Parasitemia Plasmodium chabaudi Interferon-gamma Mice Immunity Interferon parasitic diseases medicine Animals B cell Mice Knockout Immunity Cellular biology Interleukin General Medicine medicine.disease biology.organism_classification Flow Cytometry Lymphocyte Subsets Malaria Infectious Diseases medicine.anatomical_structure Immunoglobulin M Immunoglobulin G biology.protein Interleukin-2 Parasitology Female Antibody Spleen medicine.drug |
| Zdroj: | Experimental parasitology. 105(2) |
| ISSN: | 0014-4894 |
| Popis: | Cell-mediated immunity (CMI) may be important in immunity against blood-stage malaria. Accordingly, we examined the role of type 1 cytokines in the resolution of Plasmodium chabaudi adami malaria in mice genetically modified to have type 1 cytokine gene defects. Parasitemia was prolonged in double knockout (IL-2−/−, IFNγ−/−) mice compared to control mice. Despite deficiencies in γδ T cell and B cell subsets, these mice produced anti-malarial antibodies and eventually cured their infections, possibly by antibody-mediated immunity. However, because acute P. c. adami parasitemia may also be suppressed by CMI, the requirements for IL-2 and IFNγ were evaluated in mice lacking B cells and functional IL-2 or IFNγ genes. Acute malaria in JH−/−, IL-2−/− mice was prolonged, but eventually cured. In contrast, JH−/−, IFNγ−/− mice developed unremitting parasitemia. These data strongly suggest that IFNγ, but not IL-2, plays an essential role in the expression of CMI against P. c. adami infections. This finding may prove useful in developing malarial vaccines aimed at inducing CMI. Index Descriptors and Abbreviations: Plasmodium chabaudi adami (P. c. adami); malaria; rodent; TH1/2 cytokines; infectious immunity-parasites; protozoan diseases; AMI, antibody-mediated immunity; CMI, cell-mediated immunity; KO, knockout; WT, wild-type; HRP, horse-radish peroxidase; B cells, B220+/Ig+ B cells; IL, interleukin |
| Databáze: | OpenAIRE |
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