In vitrocytotoxicity of silver nanoparticles on osteoblasts and osteoclasts at antibacterial concentrations
| Autor: | Christoph E. Albers, Klaus A. Siebenrock, Regine Landmann, Frank M. Klenke, Wilhelm Hofstetter |
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| Rok vydání: | 2011 |
| Předmět: |
Silver
Materials science Biocompatibility Biomedical Engineering Metal Nanoparticles Osteoclasts Nanoparticle Microbial Sensitivity Tests In Vitro Techniques Toxicology Silver nanoparticle Mice Staphylococcus epidermidis Bone cell Animals Cytotoxic T cell Cytotoxicity Osteoblasts biology Silver Nano biology.organism_classification Anti-Bacterial Agents Mice Inbred C57BL Immunology Nuclear chemistry |
| Zdroj: | Nanotoxicology. 7:30-36 |
| ISSN: | 1743-5404 1743-5390 |
| DOI: | 10.3109/17435390.2011.626538 |
| Popis: | Nanoparticulate silver coatings for orthopaedic implants promise to decrease postoperative infection rates. However, silver-induced cytotoxicity on bone cells has not been investigated in detail. This study investigated the cytotoxic effects of silver nano- and microparticles and Ag(+) on osteoblasts (OBs) and osteoclasts (OCs) and correlated their effects with the antibacterial efficacy on Staphylococcus epidermidis. Silver nanoparticles (50 nm) exhibited strong cytotoxic effects on OBs and OCs. Weak cytotoxic effects were observed for silver microparticles (3 μm). The cytotoxicity was primarily mediated by a size-dependent release of Ag(+). Antibacterial effects occurred at Ag(+) concentrations that were 2-4 times higher than those inducing cytotoxic effects. Such adverse effects on OB and OC survival may have deleterious effects on the biocompatibility of orthopaedic implants. Our study represents an important step toward the detailed investigation of orthopaedic implant with nanoparticulate silver coatings prior to their widespread clinical usage. |
| Databáze: | OpenAIRE |
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