Arabidopsis LBP/BPI related-1 and -2 bind to LPS directly and regulate PR1 expression
| Autor: | Keiichi Watanabe, Yukio Nagano, Yutaka Kodama, Sawako Matsuzaki, Hiroyuki Tanaka, Ei’ichi Iizasa, Sayaka Iizasa |
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| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
0301 basic medicine Lipopolysaccharides Lipopolysaccharide Cell Mutant Arabidopsis 01 natural sciences Article 03 medical and health sciences chemistry.chemical_compound Immune system medicine Escherichia coli Plant Immunity Gene chemistry.chemical_classification Reactive oxygen species Multidisciplinary Membrane Glycoproteins biology Arabidopsis Proteins Glucan Endo-1 3-beta-D-Glucosidase Pathogen-Associated Molecular Pattern Molecules Membrane Proteins Blood Proteins biology.organism_classification Cell biology 030104 developmental biology medicine.anatomical_structure chemistry Immunology lipids (amino acids peptides and proteins) Bacterial outer membrane Carrier Proteins Genome Plant 010606 plant biology & botany Acute-Phase Proteins Antimicrobial Cationic Peptides |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Lipopolysaccharide (LPS) is a major constituent of the outer membrane of Gram-negative bacteria and acts as a pathogen-associated molecular pattern that triggers immune responses in both plants and animals. LPS-binding protein (LBP) and bactericidal/permeability-increasing protein (BPI), which bind to LPS and play important roles in immunity of mammals, have been well studied. However, the molecule contributing to LPS binding in plants is mostly unknown. The Arabidopsis genome carries two genes encoding LBP/BPI-related proteins which we designated as AtLBP/BPI related-1 (AtLBR-1) and AtLBP/BPI related-2 (AtLBR-2). We found that their N-terminal domains were co-purified with cell wall-derived LPS when expressed in E. coli. Since this finding implied the direct binding of AtLBRs to LPS, we also confirmed binding by using LPS-free AtLBRs and purified LPS. AtLBRs directly bind to both rough and smooth types of LPS. We also demonstrated that LPS-treated atlbr mutant Arabidopsis exhibit a significant delay of induction of defence-related gene pathogenesis-related 1 (PR1) but no other PR genes. Furthermore, LPS-treated atlbr mutants showed defects in reactive oxygen species (ROS) generation. These results demonstrate that, as well as LBP and BPI of mammals, AtLBRs also play an important role in the LPS-induced immune response of plants. |
| Databáze: | OpenAIRE |
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