Novosphingobium lotistagni sp. nov., isolated from a lotus pond
| Autor: | Ju-Han Kim, Jung-Eun Yang, Huan Trinh, Hien T. T. Ngo, Tae-Hoo Yi, MooChang Kook, Jung-Hee Kim, KyungHwa Won |
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| Rok vydání: | 2016 |
| Předmět: |
DNA
Bacterial 0106 biological sciences 0301 basic medicine Novosphingobium Ubiquinone Sequence analysis Biology medicine.disease_cause 010603 evolutionary biology 01 natural sciences Microbiology 03 medical and health sciences RNA Ribosomal 16S Republic of Korea Botany medicine Ponds Phospholipids Phylogeny Ecology Evolution Behavior and Systematics Base Composition Strain (chemistry) Fatty Acids Nucleic Acid Hybridization Novosphingobium barchaimii Sequence Analysis DNA General Medicine Ribosomal RNA 16S ribosomal RNA biology.organism_classification Bacterial Typing Techniques Sphingomonadaceae 030104 developmental biology Lotus Novosphingobium sediminicola |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 66:4729-4734 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijsem.0.001418 |
| Popis: | A Gram-staining-negative, aerobic, non-motile, rod-shaped and yellow-pigmented bacterium, designated strain THG-DN6.20T, was isolated from a lotus pond near Donghaksa temple in Daejeon, Republic of Korea. According to 16S rRNA gene sequence comparisons, strain THG-DN6.20T was found to be most closely related to Novosphingobium rosa IFO 15208T (97.6 % sequence similarity), Novosphingobium sediminicola HU1-AH51T (97.5 %) and Novosphingobium barchaimii LL02T (96.9 %). The DNA-DNA relatedness between strain THG-DN6.20T and its phylogenetically closest neighbours was below 60.0 %. The respiratory quinone and polyamine detected in strain THG-DN6.20T were ubiquinone Q-10 and spermidine, respectively. The DNA G+C content was 63.1 mol%. The major polar lipids were found to be phosphatidylethanolamine, diphosphatidylglycerol, sphingoglycolipid and phosphatidylcholine. The major fatty acids were identified as C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C14 : 0 2-OH. These data supported the affiliation of strain THG-DN6.20T to the genus Novosphingobium. Strain THG-DN6.20T could be distinguished from related species of the genus Novosphingobium by physiological and biochemical characteristics. Therefore, the novel isolate represents a novel species, for which the name Novosphingobium lotistagni sp. nov. is proposed, with THG-DN6.20T as the type strain (=KACC 18541T=CCTCC AB 2015354T). |
| Databáze: | OpenAIRE |
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