Hepatitis C virus of subtype 2l in Marseille, southeastern France
Autor: | Anne Motte, Catherine Tamalet, Patrick Borentain, Olga O. Glazunova, Sylvie Bregigeon, Laurent Chiche, D. Botta-Fridlund, Sarah Aherfi, Philippe Colson |
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Rok vydání: | 2015 |
Předmět: |
Adult
Male Genotype Hepatitis B virus DNA polymerase Hepatitis C virus Genome Viral Hepacivirus Biology Viral Nonstructural Proteins medicine.disease_cause chemistry.chemical_compound Virology medicine Humans Amino Acid Sequence NS5B Polymerase Phylogeny Aged 80 and over Sequence database Phylogenetic tree Base Sequence virus diseases Sequence Analysis DNA Hepatitis C Chronic Middle Aged RNA-Dependent RNA Polymerase digestive system diseases Infectious Diseases chemistry GenBank biology.protein RNA Viral Female France Databases Nucleic Acid |
Zdroj: | Intervirology. 58(1) |
ISSN: | 1423-0100 |
Popis: | The rate of eradication of chronic hepatitis C considerably increases with direct-acting antiviral agents, particularly hepatitis C virus (HCV) polymerase inhibitors. While implementing full-length HCV NS5B polymerase sequencing in our clinical microbiology laboratory, we identified atypical HCV sequences, classified as subtype 2l, from 2 patients. HCV-2l NS5B polymerase sequences were detected from 5 and 14 additional patients by screening our laboratory hepatitis virus sequence database and the NCBI GenBank sequence database. Phylogenetic analyses show unambiguously that all HCV-2l sequences are clustered apart from HCV 2 non-l sequences, which compose a second cluster. Mean (±SD) nucleotide identity between near full-length NS5B fragments of subtype 2l was 93.4 ± 0.8% (range: 92.4-95.1). Of note, all HCV-2l sequences obtained in our laboratory and in other centers were from serum samples collected in France. Analysis of the HCV-2l NS5B polymerase amino acid sequences at 30 positions critical for interaction with or resistance to HCV polymerase inhibitors showed specific patterns. |
Databáze: | OpenAIRE |
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