Pentosidine Effects on Human Osteoblastsin Vitro

Autor:	Fiammetta Monacelli, Roberta Sanguineti, Daniela Storace, Alberto Federici, Patrizio Odetti
Rok vydání:	2008
Předmět:	Glycation End Products Advanced medicine.medical_specialty Cell Survival Osteocalcin Receptor for Advanced Glycation End Products Osteoporosis Arginine Bone and Bones General Biochemistry Genetics and Molecular Biology Bone remodeling chemistry.chemical_compound History and Philosophy of Science Glycation Internal medicine Gene expression medicine Humans Orthopedic Procedures Receptors Immunologic Pentosidine Receptor Osteoblasts biology Reverse Transcriptase Polymerase Chain Reaction Lysine General Neuroscience Glyceraldehyde-3-Phosphate Dehydrogenases medicine.disease Endocrinology chemistry biology.protein Alkaline phosphatase Collagen Cell Division
Zdroj:	Annals of the New York Academy of Sciences. 1126:166-172
ISSN:	1749-6632 0077-8923
DOI:	10.1196/annals.1433.044
Popis:	Osteoporosis, a multifactorial and progressive skeletal metabolic disease, is characterized by low-mass density and structural deterioration of bone micro-architecture that leads to enhanced bone fragility and increased susceptibility to fractures. Recently, it has been proposed that age-related bone loss could be correlated with the glycoxidative process. The aim of the present study was to investigate the in vitro effects of pentosidine, a glycoxidative end product, on human osteoblasts (HOb). The mineralization rate, the specific bone markers (alkaline phosphatase [ALP], collagen Ialpha1 [COL Ialpha1], osteocalcin [BGP]), and the human receptor for advanced glycation end products (RAGE) gene expression have been evaluated. Pentosidine incubation of HOb caused a significant decrease in ALP, Col Ialpha1, and RAGE mRNA levels, but only the RAGE gene expression decreased with no dose dependency. Moreover, pentosidine incubation of osteoblasts hampered the formation of bone nodules. No effect was observed on BGP gene expression under all experimental conditions. Our data gives further support to a detrimental effect of AGEs on bone that leads to functional alterations of osteoblasts. This study addresses a crucial role of protein glycoxidation in the bone mineralization process. AGEs formation and accumulation in bone may be one of the first pathogenetic steps of bone remodeling in aging and in age-related diseases, leading to enhanced bone mass loss.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2ef535a4c6f90ab76f8a3211c0a84127 https://doi.org/10.1196/annals.1433.044 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.