Assembled cationic dipeptide-gold nanoparticle hybrid microspheres for electrochemical biosensors with enhanced sensitivity
| Autor: | Huimin Xue, Ziruo Li, Keqing Wang, Youqian Xu, Jie Zhao, Junbai Li, Chenlei Wang, Wei Cui, Shuo Zhang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Cholesterol oxidase
Biocompatibility Surface Properties Metal Nanoparticles Nanoparticle Biosensing Techniques 02 engineering and technology 010402 general chemistry 01 natural sciences Catalysis Biomaterials chemistry.chemical_compound Colloid and Surface Chemistry Chlorides Limit of Detection Cations Electrodes Dipeptide Cholesterol Oxidase Chemistry Cationic polymerization Reproducibility of Results Dipeptides Electrochemical Techniques Enzymes Immobilized 021001 nanoscience & nanotechnology Gold Compounds Microspheres 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Cholesterol Chemical engineering Colloidal gold Electrode Chloroauric acid lipids (amino acids peptides and proteins) Gold 0210 nano-technology |
| Zdroj: | Journal of Colloid and Interface Science. 557:628-634 |
| ISSN: | 0021-9797 |
| Popis: | Molecular assemblies of cationic dipeptide-gold nanoparticle (CDP-AuNP) hybrid microspheres were used to modify cholesterol oxidase electrodes for high-sensitivity cholesterol detection. The cationic dipeptide used here serves as a functional molecule for adsorbing chloroauric acid based on electrostatic interactions and assembly into spherical structures, providing a platform for loading gold nanoparticles (AuNPs), increasing the immobilization load of the enzyme and maintaining the activity of the enzyme as a result of excellent biocompatibility. Moreover, the CDP-AuNP modified cholesterol oxidase electrode has a higher electrocatalytic activity to cholesterol with obvious enhancement in the current response, exhibiting a current response 13 times higher than that of the controlled electrode. The outstanding biocompatibility and increased enzyme load by hybrid microspheres and the good charge-transfer ability of AuNPs in the peptide-based electrode indicate a very attractive perspective in the field of biodevices. |
| Databáze: | OpenAIRE |
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