Allergen-specific helper T cell response in patients with cow's milk allergy: simultaneous analysis of proliferation and cytokine production by carboxyfluorescein succinimidyl ester dilution assay

Autor: Yasuto Kondo, R. Komatsubara, Yoichi Nakajima, Makiko Kawamura, Atsuo Urisu, K. Yamada, Reiko Tokuda, Michiko Kakami, Ikuya Tsuge
Rok vydání: 2006
Předmět:
Time Factors
medicine.medical_treatment
T cell
Immunology
Succinimides
Milk allergy
Immunologic Tests
Biology
Lymphocyte Activation
Statistics
Nonparametric
Interferon-gamma
chemistry.chemical_compound
Immune Tolerance
medicine
Humans
Immunology and Allergy
Interferon gamma
Cells
Cultured
health care economics and organizations
Interleukin 4
Cell Proliferation
Fluorescent Dyes
Chi-Square Distribution
Dose-Response Relationship
Drug
Ionomycin
Carboxyfluorescein succinimidyl ester
T-Lymphocytes
Helper-Inducer
Allergens
Flow Cytometry
Fluoresceins
Milk Proteins
medicine.disease
Stimulation
Chemical
humanities
Interleukin-10
Interleukin 10
Tolerance induction
Cytokine
medicine.anatomical_structure
chemistry
Case-Control Studies
Cytokines
Tetradecanoylphorbol Acetate
Interleukin-4
Milk Hypersensitivity
Food Hypersensitivity
medicine.drug
Zdroj: Clinical & Experimental Allergy. 36:1538-1545
ISSN: 1365-2222
0954-7894
Popis: Summary Background The role of antigen-specific T cells in the allergic reaction to cow's milk or in tolerance induction is not yet fully understood. Objective This study was designed to analyse both cow's milk protein (CMP)-specific T cell proliferation and cytokine production simultaneously in children with cow's milk allergy (CMA) in comparison with subjects with various allergic backgrounds. Methods Carboxyfluorescein succinimidyl ester was used to detect cow's milk-specific T cells by flow cytometry. The intra-cytoplasmic cytokine production of these antigen-specific T cells was also analysed. Results Significant differences of both CMP-specific CD4+ cell proliferation and cytokine production between CMA and non-allergic children were observed. While the proliferative responses of children who recently outgrew CMA were not significantly different from those of patients, the patterns of cytokine production were similar to those of non-allergic children. Conclusion These results suggest that the presence of CMP-specific T cell clones per se does not produce CMA, but that the T-helper type 2-skewed pattern of those T cells is associated with adverse reactions. Although it is not possible to distinguish between individual patients with and without CMA on the basis of CFSE assays, these results contribute to the understanding of the pathogenesis and tolerance induction of CMA.
Databáze: OpenAIRE
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