Allergen-specific helper T cell response in patients with cow's milk allergy: simultaneous analysis of proliferation and cytokine production by carboxyfluorescein succinimidyl ester dilution assay
Autor: | Yasuto Kondo, R. Komatsubara, Yoichi Nakajima, Makiko Kawamura, Atsuo Urisu, K. Yamada, Reiko Tokuda, Michiko Kakami, Ikuya Tsuge |
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Rok vydání: | 2006 |
Předmět: |
Time Factors
medicine.medical_treatment T cell Immunology Succinimides Milk allergy Immunologic Tests Biology Lymphocyte Activation Statistics Nonparametric Interferon-gamma chemistry.chemical_compound Immune Tolerance medicine Humans Immunology and Allergy Interferon gamma Cells Cultured health care economics and organizations Interleukin 4 Cell Proliferation Fluorescent Dyes Chi-Square Distribution Dose-Response Relationship Drug Ionomycin Carboxyfluorescein succinimidyl ester T-Lymphocytes Helper-Inducer Allergens Flow Cytometry Fluoresceins Milk Proteins medicine.disease Stimulation Chemical humanities Interleukin-10 Interleukin 10 Tolerance induction Cytokine medicine.anatomical_structure chemistry Case-Control Studies Cytokines Tetradecanoylphorbol Acetate Interleukin-4 Milk Hypersensitivity Food Hypersensitivity medicine.drug |
Zdroj: | Clinical & Experimental Allergy. 36:1538-1545 |
ISSN: | 1365-2222 0954-7894 |
Popis: | Summary Background The role of antigen-specific T cells in the allergic reaction to cow's milk or in tolerance induction is not yet fully understood. Objective This study was designed to analyse both cow's milk protein (CMP)-specific T cell proliferation and cytokine production simultaneously in children with cow's milk allergy (CMA) in comparison with subjects with various allergic backgrounds. Methods Carboxyfluorescein succinimidyl ester was used to detect cow's milk-specific T cells by flow cytometry. The intra-cytoplasmic cytokine production of these antigen-specific T cells was also analysed. Results Significant differences of both CMP-specific CD4+ cell proliferation and cytokine production between CMA and non-allergic children were observed. While the proliferative responses of children who recently outgrew CMA were not significantly different from those of patients, the patterns of cytokine production were similar to those of non-allergic children. Conclusion These results suggest that the presence of CMP-specific T cell clones per se does not produce CMA, but that the T-helper type 2-skewed pattern of those T cells is associated with adverse reactions. Although it is not possible to distinguish between individual patients with and without CMA on the basis of CFSE assays, these results contribute to the understanding of the pathogenesis and tolerance induction of CMA. |
Databáze: | OpenAIRE |
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