Cystatin C expression is associated with granule cell dispersion in epilepsy
| Autor: | Leo Paljärvi, Reetta Kälviäinen, Irina Alafuzoff, Asla Pitkänen, Arto Immonen, Leena Jutila, Matti Vapalahti, Kari Karkola, Terhi J. Pirttilä, Anni Manninen, Jari Nissinen, Esa Mervaala |
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| Rok vydání: | 2005 |
| Předmět: |
Adult
Male medicine.medical_specialty Adolescent Cell Count Neural Cell Adhesion Molecule L1 Biology Epileptogenesis Rats Sprague-Dawley Epilepsy Cell Movement Internal medicine Glial Fibrillary Acidic Protein medicine Animals Humans Cystatin C Aged Aged 80 and over Neurons Cell Death Dentate gyrus Neurogenesis Neurodegeneration Electroencephalography Middle Aged medicine.disease Cystatins Immunohistochemistry Temporal Lobe Rats Granule cell dispersion Disease Models Animal Endocrinology Epilepsy Temporal Lobe Gene Expression Regulation nervous system Neurology Phosphopyruvate Hydratase Dentate Gyrus Sialic Acids biology.protein Female Neurology (clinical) Cystatin |
| Zdroj: | Annals of Neurology. 58:211-223 |
| ISSN: | 0364-5134 |
| Popis: | Human temporal lobe epilepsy (TLE) is associated with cellular alterations (eg, hilar cell death, neurogenesis, and granule cell dispersion) in the dentate gyrus but their underlying molecular mechanism are not known. We previously demonstrated increased expression of cystatin C, a protease inhibitor linked to both neurodegeneration and neurogenesis, during epileptogenesis in the rat hippocampus. Here, we investigated cystatin C expression in the dentate gyrus in chronic epilepsy and its association with neuronal loss and neurogenesis. In both rats with epilepsy and human patients with TLE, cystatin C expression was increased in glial cells in the molecular layer of the dentate gyrus, being most prominent in cases with granule cell dispersion. In patients with TLE, high cystatin C expression associated with greater numbers of polysialylated neural cell adhesion molecule-positive newborn cells in the molecular layer, although the overall number was decreased, indicating that the newborn cells migrate to abnormal locations in the epileptic dentate gyrus. These data thus demonstrate that cystatin C expression is altered during the chronic phase of epilepsy and suggest that cystatin C plays a role in network reorganization in the epileptic dentate gyrus, especially in granule cell dispersion and guidance of migrating newborn granule cells. |
| Databáze: | OpenAIRE |
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