Effective MSTN Gene Knockout by AdV-Delivered CRISPR/Cas9 in Postnatal Chick Leg Muscle
| Autor: | Jin Mei Ding, Zhong Xu, Lingyu Yang, He Meng, Hao Zhou, Chuan He, Zheng Yuming, Chao Qin, Cheng Xiao Han, Huai Xi Luo, Ke Xu, Fisayo T. Akinyemi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
skeletal muscle tissue Cellular differentiation knockout Myostatin transcriptome sequencing Article Catalysis Muscle hypertrophy Animals Genetically Modified Inorganic Chemistry Transcriptome lcsh:Chemistry Gene Knockout Techniques 03 medical and health sciences 0302 clinical medicine Gene expression Animals CRISPR Physical and Theoretical Chemistry Molecular Biology Gene lcsh:QH301-705.5 CRISPR/Cas9 Spectroscopy Gene knockout Gene Editing postnatal gene editing biology Gene Expression Profiling Organic Chemistry General Medicine differentially expressed gene Computer Science Applications Cell biology chick 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 myostatin biology.protein CRISPR-Cas Systems Chickens 030217 neurology & neurosurgery |
| Zdroj: | International Journal of Molecular Sciences Volume 21 Issue 7 International Journal of Molecular Sciences, Vol 21, Iss 2584, p 2584 (2020) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms21072584 |
| Popis: | Muscle growth and development are important aspects of chicken meat production, but the underlying regulatory mechanisms remain unclear and need further exploration. CRISPR has been used for gene editing to study gene function in mice, but less has been done in chick muscles. To verify whether postnatal gene editing could be achieved in chick muscles and determine the transcriptomic changes, we knocked out Myostatin (MSTN), a potential inhibitor of muscle growth and development, in chicks and performed transcriptome analysis on knock-out (KO) muscles and wild-type (WT) muscles at two post-natal days: 3d (3-day-old) and 14d (14-day-old). Large fragment deletions of MSTN (> 5 kb) were achieved in all KO muscles, and the MSTN gene expression was significantly downregulated at 14d. The transcriptomic results indicated the presence of 1339 differentially expressed genes (DEGs) between the 3d KO and 3d WT muscles, as well as 597 DEGs between 14d KO and 14d WT muscles. Many DEGs were found to be related to cell differentiation and proliferation, muscle growth and energy metabolism. This method provides a potential means of postnatal gene editing in chicks, and the results presented here could provide a basis for further investigation of the mechanisms involved in muscle growth and development. |
| Databáze: | OpenAIRE |
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