Trichloroethene degradation in a two-step system bymethylosinus trichosporium OB3b. Optimization of system performance: Use of formate and methane
| Autor: | J.E.T. van Hylckama Vlieg, K.J Ganzeveld, E.M Sipkema, W. de Koning, Dick B. Janssen, Antonie A. C. M. Beenackers |
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| Přispěvatelé: | Groningen Biomolecular Sciences and Biotechnology |
| Rok vydání: | 1999 |
| Předmět: |
Inorganic chemistry
Bioengineering Environmental pollution Electron donor Applied Microbiology and Biotechnology Methylococcaceae Methane MIXED CULTURE Microbiology chemistry.chemical_compound transformation capacity Bioreactor Formate Methylosinus trichosporium OB3b two-step bioreactor system biology Chemistry 2-STAGE METHANOTROPHIC BIOREACTOR Biodegradation biology.organism_classification trichloroethene SEQUENCING BIOFILM REACTOR BIODEGRADATION KINETICS TCE DEGRADATION CHLORINATED-HYDROCARBON DEGRADATION Degradation (geology) EXPANDED-BED BIOREACTORS OXIDIZING BACTERIA ALIPHATIC-HYDROCARBONS PRODUCT TOXICITY Biotechnology |
| Zdroj: | Biotechnology and Bioengineering, 63(1), 56-68. Wiley-Blackwell |
| ISSN: | 1097-0290 0006-3592 |
| DOI: | 10.1002/(sici)1097-0290(19990405)63:1<56::aid-bit6>3.0.co;2-f |
| Popis: | The breakdown of dissolved TCE in a two-step bioremediation system is described. In the first reactor, the organism Methylosinus trichosporium OB3b is grown; in the second reactor, consisting of three 17-L column reactors in series, the cells degrade TCE. A special design allowed both for the addition of air (uG,s = 0.01-0. 04 mm s-1) in the conversion reactor to prevent oxygen limitation while minimizing stripping of TCE, and for the use of methane as exogenous electron donor. In two-step systems presented thus far, only formate was used (excess, 20 mM). We found formate additions could be reduced by 75% (15 degrees C), whereas small amounts of methane (0.02-0.04 mol CH4/g cells) could replace formate and led to equally optimal results. Example calculations show that up to 90% reduction in operating cost of chemicals can be obtained by using methane instead of formate. A model was developed to describe each of the conditions studied: excess formate and optimal methane addition, suboptimal formate addition and suboptimal methane addition. Using parameters obtained from independent batch experiments, the model gives a very good description of the overall TCE conversion in the two-step system. The system presented is flexible (oxygen/methane addition) and can easily be scaled up for field application. The model provides a tool for the design of an effective and low-cost treatment system based on methane addition in the conversion reactor. |
| Databáze: | OpenAIRE |
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