Epithelial cells are the major source of biologically active granulocyte macrophage colony-stimulating factor in human endometrium
| Autor: | G. Giacomini, P.G. Satyaswaroop, Pierluigi Strippoli, S.S. Tabibzadeh, V.M. Jasonni, L. Bonsi, L. Vitale, G. P. Bagnara |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Adult
medicine.medical_specialty Stromal cell medicine.medical_treatment Blotting Western Biology Endometrium Epithelium Mice Cytokeratin Antigen Neutralization Tests Pregnancy Culture Techniques Internal medicine medicine Animals Humans Macrophage Growth factor Rehabilitation Antibodies Monoclonal Granulocyte-Macrophage Colony-Stimulating Factor Obstetrics and Gynecology Epithelial Cells Middle Aged Immunohistochemistry Molecular biology Endocrinology Granulocyte macrophage colony-stimulating factor medicine.anatomical_structure Cytokine Reproductive Medicine Culture Media Conditioned Biological Assay Female medicine.drug |
| Zdroj: | Human Reproduction. 10:3259-3263 |
| ISSN: | 1460-2350 0268-1161 |
| DOI: | 10.1093/oxfordjournals.humrep.a135899 |
| Popis: | Granulocyte macrophage colony-stimulating factor (GM-CSF) has emerged as an important growth factor for trophoblast and other placental cells, leading to improved placental functioning and fetal survival. Recent observations have indicated that GM-CSF is synthesized by epithelial cells in the murine pregnant and non-pregnant uterus. In this study, the production of GM-CSF by cells derived from human endometrium is assessed using a sensitive bioassay and specific neutralization of the cytokine bioactivity with a monoclonal antibody to GM-CSF. Originally, GM-CSF was assayed in the culture supernatants of explant cultures of human endometria. Concentrations of GM-CSF up to 4440 pg/ml were detected. Subsequently, enriched epithelial cell cultures were prepared from glands isolated from human endometrium. The purity of epithelial cultures was demonstrated by the expression of cytokeratin, a weak immunoreactivity for vimentin and a lack of immunoreactivity for leukocyte common antigen, CD68, a macrophage-specific protein and endothelial marker (factor VIII-related antigens). Detected concentrations of GM-CSF were as high as 18,800 pg/ml. Furthermore, pure epithelial cells of a neoplastic endometrial cell line ECC1 secreted GM-CSF, confirming the ability of endometrial epithelial cells to secrete this cytokine. The immunostaining of dated endometria from proliferative and secretory phases showed primarily that epithelial cells, and to a lesser extent stromal cells, exhibited immunoreactivity for GM-CSF. A Western blot analysis, performed to validate the immunohistochemical data, confirmed the presence of an immunoreactive gene product for GM-CSF in human endometrium throughout the menstrual cycle. These findings indicate that human endometrium synthesizes GM-CSF and that epithelial cells are a major contributor to its production. |
| Databáze: | OpenAIRE |
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