Cerium oxide nanomaterial with dual antioxidative scavenging potential: Synthesis and characterization
| Autor: | Udit Kumar, Tamil S. Sakthivel, Sushant Singh, Balaashwin Babu, David Gittess, Sudipta Seal |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Cerium oxide
Biomedical Engineering 02 engineering and technology Antioxidants Cell Line Phosphates Nanomaterials Biomaterials Superoxide dismutase 03 medical and health sciences Microscopy Electron Transmission Materials Testing Animals Humans Scavenging 030304 developmental biology Ions chemistry.chemical_classification 0303 health sciences Reactive oxygen species biology Superoxide Dismutase Chemistry Cerium Hydrogen Peroxide Catalase 021001 nanoscience & nanotechnology Combinatorial chemistry Nanostructures biology.protein Nanoparticles Reactive Oxygen Species 0210 nano-technology |
| Zdroj: | Journal of Biomaterials Applications. 36:834-842 |
| ISSN: | 1530-8022 0885-3282 |
| DOI: | 10.1177/08853282211013451 |
| Popis: | Many studies have linked reactive oxygen species (ROS) to various diseases. Biomedical research has therefore sought a way to control and regulate ROS produced in biological systems. In recent years, cerium oxide nanoparticles (nanoceria, CNPs) have been pursued due to their ability to act as regenerative ROS scavengers. In particular, they are shown to have either superoxide dismutase (SOD) or catalase mimetic (CAT) potential depending on the ratio of Ce3+/Ce4+ valence states. Moreover, it has been demonstrated that SOD mimetic activity can be diminished by the presence of phosphate, which can be a problem given that many biological systems operate in a phosphate-rich environment. Herein, we report a CNP formulation with both SOD and catalase mimetic activity that is preserved in a phosphate-rich media. Characterization demonstrated a highly dispersed, stable solution of uniform-sized, spherical-elliptical shaped CNP of 12 ± 2 nm, as determined through dynamic light scattering, zeta potential, and transmission electron microscopy. Mixed valence states of Ce ions were observed via UV/Visible spectroscopy and XPS (Ce3+/Ce4+ > 1) (Ce3+∼ 62%). X-ray diffraction and XPS confirmed the presence of oxygen-deficient cerium oxide (CeO2-x) particles. Finally, the CNP demonstrated very good biocompatibility and efficient reduction of hydrogen peroxide under in-vitro conditions. |
| Databáze: | OpenAIRE |
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