USDA FSIS, FDA BAM, AOAC, and ISO Culture Methods BD BBL CHROMagar Listeria Media
| Autor: | Vicki Ritter, Krista Sturm, Patty Warns, Susan Kircher, Nancy Dick |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Meat
medicine.disease_cause Analytical Chemistry Food and drug administration food Listeria monocytogenes Cheese Salmon medicine Animals Environmental Chemistry Food science United States Department of Agriculture Pharmacology biology United States Food and Drug Administration business.industry Reproducibility of Results food and beverages Brie cheese Lettuce Reference Standards biology.organism_classification Food safety United States food.food Culture Media Solutions Smoked salmon Smoked fish Food Microbiology Listeria Cattle Indicators and Reagents business Agronomy and Crop Science Food Science Food contaminant |
| Zdroj: | Journal of AOAC INTERNATIONAL. 92:1105-1117 |
| ISSN: | 1944-7922 1060-3271 |
| DOI: | 10.1093/jaoac/92.4.1105 |
| Popis: | BBL CHROMagar Listeria Media (CL) was evaluated for detection of Listeria monocytogenes in raw ground beef, smoked salmon, lettuce, and Brie cheese. The recovery of L. monocytogenes on CL was compared to the U.S. Food and Drug Administration (FDA) Bacteriological Analytical Manual (BAM), U.S. Department of Agriculture (USDA) Food Safety and Inspection Service (FSIS), AOAC, and International Organization for Standardization (ISO) reference-plated media using the recommended pre-enrichments and selective enrichments. Of the 265 food samples tested, 140 were tested using BAM, USDA, or AOAC methods and 125 were tested using ISO methods. CL produced comparable results with the reference methods on all matrixes with a sensitivity of 99.3 and a specificity of 100. No false negatives were found in testing the food matrixes. There was no statistical difference in recovery based on Chi-square analysis. Known isolates were evaluated, and CL had a sensitivity and specificity of 100. The results of this study demonstrate that CL is an effective medium for the recovery and detection of L. monocytogenes in raw ground beef, smoked salmon, lettuce, and Brie cheese using FDA BAM, USDA FSIS, AOAC, and ISO culture methods. |
| Databáze: | OpenAIRE |
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