Rice yellow stunt virus activates polyamine biosynthesis to promote viral propagation in insect vectors by disrupting ornithine decarboxylase antienzyme function
| Autor: | Tianbao Zeng, Zongwen Wang, Yuemin Zheng, Yunjie Xie, Hanbin Lin, Xiaofeng Zhang, Huanqin Wang, Taiyun Wei |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
viruses polyamines Spermine Grasshoppers Biology rice yellow stunt virus General Biochemistry Genetics and Molecular Biology Virus Ornithine decarboxylase Plant Viruses 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine plant virus Biosynthesis leafhopper Animals General Environmental Science Viral matrix protein Oryza Ornithine Decarboxylase Inhibitors Cell biology Insect Vectors Spermidine 030104 developmental biology chemistry 030220 oncology & carcinogenesis Putrescine viral infection General Agricultural and Biological Sciences Polyamine Research Paper |
| Zdroj: | Science China. Life Sciences |
| ISSN: | 1869-1889 |
| Popis: | Intracellular polyamines (putrescine, spermidine, and spermine) have emerged as important molecules for viral infection; however, how viruses activate polyamines biosynthesis to promote viral infection remains unclear. Ornithine decarboxylase 1 (ODC1) and its antienzyme 1 (OAZ1) are major regulators of polyamine biosynthesis in animal cells. Here, we report that rice yellow stunt virus (RYSV), a plant rhabdovirus, could activate putrescine biosynthesis in leafhoppers to promote viral propagation by inhibiting OAZ1 expression. We observed that the reduction of putrescine biosynthesis by treatment with difluormethylornithine (DFMO), a specific nontoxic inhibitor of ODC1, or with in vitro synthesized dsRNAs targeting ODC1 mRNA could inhibit viral infection. In contrast, the supplement of putrescine or the increase of putrescine biosynthesis by treatment with dsRNAs targeting OAZ1 mRNA could facilitate viral infection. We further determined that both RYSV matrix protein M and ODC1 directly bind to the ODC-binding domain at the C-terminus of OAZ1. Thus, viral propagation in leafhoppers would decrease the ability of OAZ1 to target and mediate the degradation of ODC1, which finally activates putrescine production to benefit viral propagation. This work reveals that polyamine-metabolizing enzymes are directly exploited by a vector-borne virus to increase polyamine production, thereby facilitating viral infection in insect vectors. Electronic Supplementary Material Supplementary material is available for this article at 10.1007/s11427-020-1846-8 and is accessible for authorized users. |
| Databáze: | OpenAIRE |
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