The cytoplasmic tail of HIV-1 gp160 contains regions that associate with cellular membranes
| Autor: | Phillip W. Berman, Donald Dowbenko, O K Haffar |
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| Rok vydání: | 1991 |
| Předmět: |
Signal peptide
Cytoplasm Recombinant Fusion Proteins Protein Sorting Signals Biology Transfection Cell Line HIV Envelope Protein gp160 Viral Proteins Viral Envelope Proteins Viral envelope Virology Humans Protein Precursors Lipid bilayer chemistry.chemical_classification Cell Membrane Gene Products env Biological Transport Fusion protein Cell biology Transmembrane domain Membrane chemistry Biochemistry HIV-1 Glycoprotein Plasmids |
| Zdroj: | Virology. 180:439-441 |
| ISSN: | 0042-6822 |
| Popis: | The HIV-1 envelope glycoprotein gpl60 associates with cellular membranes via a discrete transmembrane domain. Unlike other retroviral envelope proteins, however, gp160 also forms a secondary association with the lipid bilayer mediated by one or more regions located in the cytoplasmic tail. We have expressed the full cytoplasmic tail sequence of gpl60, as a fusion protein with the HSV-1 glycoprotein D signal sequence, transiently in a human embryonic kidney cell line. Our results show that in the absence of any defined transmembrane domain or stop transfer sequence, the protein corresponding to the cytoplasmic tail of HIV-1 gpl60 formed stable interactions with cellular membranes that mediated its export to the cell surface. |
| Databáze: | OpenAIRE |
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