Layer-specific expression of extracellular matrix molecules in the mouse somatosensory and piriform cortices
| Autor: | Motoi Okamoto, Takeshi Ishihara, Shunsuke Suemitsu, Hiroshi Ueno, Yosuke Matsumoto, Kenta Wani, Naoya Kitamura, Shinji Murakami |
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| Rok vydání: | 2019 |
| Předmět: |
HABP
hyaluronic acid binding protein 0301 basic medicine Perineuronal nets ChABC chondroitinase ABC CNS central nervous system Piriform cortex Somatosensory system Article lcsh:RC321-571 Extracellular matrix 03 medical and health sciences WFA Wisteria floribunda agglutinin 0302 clinical medicine Neurocan ECM extracellular cellular matrix Brevican HA hyaluronic acid lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Aggrecan biology Chemistry General Neuroscience Perineuronal net a.u. arbitrary units Hapln1 hyaluronan and proteoglycan link protein 1 PNN perineuronal ntes Wisteria floribunda biology.organism_classification Somatosensory cortex Cell biology 030104 developmental biology CSPG chondroitin sulfate proteoglycans Proteoglycans 030217 neurology & neurosurgery |
| Zdroj: | IBRO Reports, Vol 6, Iss, Pp 1-17 (2019) IBRO Reports |
| ISSN: | 2451-8301 |
| DOI: | 10.1016/j.ibror.2018.11.006 |
| Popis: | Highlights • Extracellular matrix (ECM) molecules are distributed as diffuse ECM or concentrated meshwork structured perineuronal nets. • ECM molecules differ in constituent elements and expression in a region- and layer-dependent manner. • Region-specific ECM molecule expression may be associated with region-specific plasticity and vulnerability functions. • Many ECM molecules showed higher expression in L1 than in the other layers of the piriform cortex. • Changes in ECM molecules due to aging significantly differed in each brain region. In the developing central nervous system (CNS), extracellular matrix (ECM) molecules have regulating roles such as in brain development, neural-circuit maturation, and synaptic-function control. However, excluding the perineuronal net (PNN) area, the distribution, constituent elements, and expression level of granular ECM molecules (diffuse ECM) present in the mature CNS remain unclear. Diffuse ECM molecules in the CNS share the components of PNNs and are likely functional. As cortical functions are greatly region-dependent, we hypothesized that ECM molecules would differ in distribution, expression level, and components in a region- and layer-dependent manner. We examined the layer-specific expression of several chondroitin sulfate proteoglycans (aggrecan, neurocan, and brevican), tenascin-R, Wisteria floribunda agglutinin (WFA)-positive molecules, hyaluronic acid, and link protein in the somatosensory and piriform cortices of mature mice. Furthermore, we investigated expression changes in WFA-positive molecules due to aging. In the somatosensory cortex, PNN density was particularly high at layer 4 (L4), but not all diffuse ECM molecules were highly expressed at L4 compared to the other layers. There was almost no change in tenascin-R and hyaluronic acid in any somatosensory-cortex layer. Neurocan showed high expression in L1 of the somatosensory cortex. In the piriform cortex, many ECM molecules showed higher expression in L1 than in the other layers. However, hyaluronic acid showed high expression in deep layers. Here, we clarified that ECM molecules differ in constituent elements and expression in a region- and layer-dependent manner. Region-specific expression of ECM molecules is possibly related to functions such as region-specific plasticity and vulnerability. |
| Databáze: | OpenAIRE |
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