A rapid collection of yet unknown ammonia oxidizers in pure culture from activated sludge
| Autor: | Hirotsugu Fujitani, Satoshi Tsuneda, Takuma Abe, Norisuke Ushiki |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Environmental Engineering Denitrification 03 medical and health sciences chemistry.chemical_compound Ammonia Nitrosomonas Waste Management and Disposal Phylogeny Water Science and Technology Civil and Structural Engineering Growth medium Bacteria Sewage biology Waste management Ecological Modeling biology.organism_classification Nitrification Pollution 030104 developmental biology Activated sludge Wastewater Biochemistry chemistry Sewage treatment Oxidation-Reduction |
| Zdroj: | Water Research. 108:169-178 |
| ISSN: | 0043-1354 |
| DOI: | 10.1016/j.watres.2016.10.070 |
| Popis: | Nitrification is an important reaction in the biological nitrogen removal process in wastewater treatment plants (WWTPs). As ammonia-oxidizing microbes are slow-growing and sensitive to environmental factors such as free ammonia, pure strains are hard to obtain, preventing our understanding of their physiological characteristics. To conquer this hurdle, we report a high-throughput isolation technique based on scattering signatures, which exploits the tendency of many ammonia-oxidizing bacteria (AOB) to form microcolonies in activated sludge. The AOB microcolonies were directly sorted from the activated sludge without long incubation and enrichment bias, and were sequentially inoculated into 96-well microtiter plates containing growth medium. Phylogenetic analysis of the pure strains isolated in this study revealed a deeply branching and unrecognized lineage and diversity within the genus Nitrosomonas, beyond our expectation. |
| Databáze: | OpenAIRE |
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