Th17 Cells Contribute to Viral Replication in Coxsackievirus B3-Induced Acute Viral Myocarditis
| Autor: | Yu-Hua Liao, Qiong-Wen Lin, Ai-Lin Cao, Xiang Cheng, Jin-Ping Wang, Ji-Hua Dong, Min Wang, Miao Yu, Jing Yuan, Jing-Hui Zhang, Xian Yu, He-Ping Guo |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Male
Viral Myocarditis viruses medicine.medical_treatment Blotting Western Immunology Coxsackievirus Infections Cell Separation Biology Virus Replication Flow cytometry Proinflammatory cytokine Mice Downregulation and upregulation T-Lymphocyte Subsets medicine Animals Immunology and Allergy RNA Messenger Mice Inbred BALB C medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction Interleukin-17 T-Lymphocytes Helper-Inducer Flow Cytometry Virology Enterovirus B Human Blot Myocarditis Cytokine Viral replication cardiovascular system CD8 |
| Zdroj: | The Journal of Immunology. 185:4004-4010 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.1001718 |
| Popis: | Acute viral myocarditis (AVMC) is characterized by virus-triggered myocardial inflammation, and Coxsackievirus B3 (CVB3) is the primary pathogen. We previously proved that Th17 cells, besides having proinflammatory effects, were involved in AVMC by enhancing humoral response. However, the relationship between Th17 cells and CVB3 replication remains unknown. In this experiment, we infected BALB/c mice with CVB3 for establishing AVMC models and then found that, with the increase of viral replication, the expressions of splenic Th17 cells, serum IL-17, and cardiac IL-17 mRNA were elevated significantly, accompanied by the progressive cardiac injuries of AVMC. Furthermore, on day 5, the peak time for viral replication, correlation was positive between cardiac IL-17 mRNA and CVB3 RNA (correlation index = 0.835; p < 0.01). Although the expressions of Th1 and CD8+ T cells, which could secrete the antiviral cytokine IFN-γ and damage the heart, were also elevated, along with Th17 cells, in AVMC, the neutralization of IL-17 further upregulated the percentages of splenic Th1 and CD8+ T cells and the levels of cardiac IFN-γ mRNA. The cardiac pathological changes were obviously improved after neutralization, with reduced viral replication followed by decreases in the cardiac inflammatory cytokines IL-17, TNF-α, and IL-1β. These data suggest that Th17 cells contribute to CVB3 replication in AVMC, and that IL-17 might be an important target for regulating the balance of antiviral immunities. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|