Molecular Mechanism of the ATF6α/S1P/S2P Signaling Pathway in Hippocampal Neuronal Apoptosis in SPS Rats
| Autor: | Liang Han, Yanhao Xu, Yuxiu Shi |
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| Rok vydání: | 2021 |
| Předmět: |
Male
medicine.medical_specialty education Apoptosis Hippocampal formation CHOP Hippocampus Stress Disorders Post-Traumatic Cellular and Molecular Neuroscience chemistry.chemical_compound Memory Internal medicine AEBSF medicine Hippocampus (mythology) Animals Rats Wistar Caspase 12 Heat-Shock Proteins Neurons TUNEL assay fungi Serine Endopeptidases General Medicine Endoplasmic Reticulum Stress Activating Transcription Factor 6 Rats Blot Endocrinology chemistry Proprotein Convertases Signal transduction Transcription Factor CHOP Signal Transduction |
| Zdroj: | Journal of molecular neuroscience : MN. 71(12) |
| ISSN: | 1559-1166 |
| Popis: | Apoptosis of hippocampal neurons is one of the mechanisms of hippocampal atrophy in posttraumatic stress disorder (PTSD), and it is also an important cause of memory impairment in PTSD patients. Endoplasmic reticulum stress (ERS) mediated by activated transcription factor 6α (ATF6α)/site 1 protease (S1P)/S2P is involved in cell apoptosis, but it is not clear whether it is involved in hippocampal neuron apoptosis caused by PTSD. A PTSD rat model was constructed by the single prolonged stress (SPS) method. The study was divided into three parts. Experiment 1 included the control group, SPS 1 d group, SPS 7 d group, and SPS 14 d group. Experiment 2 included the control group, SPS 7 d group, SPS 7 d + AEBSF group, and control + AEBSF group. (4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) is an ATF6α pathway inhibitor). Experiment 3 included the control group, SPS 4 d group, SPS 4 d + AEBSF group, and control + AEBSF group. The protein and mRNA expression levels of ATF6α, glucose-regulated protein (GRP78), S1P, S2P, C/EBP homologous protein (CHOP), and caspase-12 in the hippocampus of PTSD rats were detected by immunohistochemistry, Western blotting and qRT-PCR. Apoptosis of hippocampal neurons was detected by TUNEL staining. In experiment 1, the protein and mRNA expression of ATF6α and GRP78 increased gradually in the SPS 1 d group and the SPS 7 d group but decreased in the SPS 14 d group (P |
| Databáze: | OpenAIRE |
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