Human apolipoprotein A-II associates with triglyceride-rich lipoproteins in plasma and impairs their catabolism
| Autor: | A.-D. Kalopissis, Michèle Chabert, Virginie Pautre, Sonia Dugué-Pujol, Nhuan Tran Quang, Xavier Rousset, Jean Chambaz, Danièle Pastier |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Very low-density lipoprotein medicine.medical_specialty Apolipoprotein B Lipoproteins Mice Transgenic QD415-436 Lipoproteins VLDL transgenic mice Biochemistry plasma residence time Mice chemistry.chemical_compound Endocrinology Internal medicine medicine Animals Humans Intestinal Mucosa Triglycerides Mice Knockout Lipoprotein lipase Triglyceride biology Hypertriglyceridemia Cell Biology medicine.disease Recombinant Proteins postprandial hypertriglyceridemia apolipoprotein A-II knockout mice Mice Inbred C57BL very low density lipoprotein chemistry Hyperglycemia biology.protein Female chylomicrons lipids (amino acids peptides and proteins) Hepatic lipase Apolipoprotein C2 Apolipoprotein A-II Chylomicron |
| Zdroj: | Journal of Lipid Research, Vol 47, Iss 12, Pp 2631-2639 (2006) |
| ISSN: | 0022-2275 |
| Popis: | Postprandial hypertriglyceridemia and low plasma HDL levels, which are principal features of the metabolic syndrome, are displayed by transgenic mice expressing human apolipoprotein A-II (hapoA-II). In these mice, hypertriglyceridemia results from the inhibition of lipoprotein lipase and hepatic lipase activities by hapoA-II carried on VLDL. This study aimed to determine whether the association of hapoA-II with triglyceride-rich lipoproteins (TRLs) is sufficient to impair their catabolism. To measure plasma TRL residence time, intestinal TRL production was induced by a radioactive oral lipid bolus. Radioactive and total triglyceride (TG) were rapidly cleared in control mice but accumulated in plasma of transgenic mice, in relation to hapoA-II concentration. Similar plasma TG accumulations were measured in transgenic mice with or without endogenous apoA-II expression. HapoA-II (synthesized in liver) was detected in chylomicrons (produced by intestine). The association of hapoA-II with TRL in plasma was further confirmed by the absence of hapoA-II in chylomicrons and VLDL of transgenic mice injected with Triton WR 1339, which prevents apolipoprotein exchanges. We show that the association of hapoA-II with TRL occurs in the circulation and induces postprandial hypertriglyceridemia. |
| Databáze: | OpenAIRE |
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