Influence of Wilms' tumor suppressor gene WT1 on bovine Sertoli cells polarity and tight junctions via non-canonical WNT signaling pathway
| Autor: | S.O. Adeniran, Peng Zheng, Xue Wang, Guixue Zhang, Mingjun Ma, Elikanah Olusayo Adegoke, Han Zhang, Fushuo Huang |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Tumor suppressor gene CDC42 Occludin Tight Junctions 03 medical and health sciences 0302 clinical medicine Food Animals WNT4 Animals Small Animals Spermatogenesis WT1 Proteins Wnt Signaling Pathway Blood-Testis Barrier Cells Cultured Gene knockdown 030219 obstetrics & reproductive medicine Sertoli Cells Tight junction urogenital system Equine Chemistry 0402 animal and dairy science Wnt signaling pathway Cell Polarity 04 agricultural and veterinary sciences 040201 dairy & animal science Cell biology Animals Newborn Animal Science and Zoology Cattle Signal transduction tissues |
| Zdroj: | Theriogenology. 138 |
| ISSN: | 1879-3231 |
| Popis: | Sertoli cells (SCs) are polarized epithelial cells and provide a microenvironment for the development of germ cells (GCs). The Wilms' tumor suppressor gene WT1 which support spermatogenesis is expressed explicitly in SCs. This study investigated the effect of WT1 on the polarity and blood-testis barrier (BTB) formation of bovine SCs in order to provide theoretical and practical bases for the spermatogenic process in mammals. In this study, newborn calf SCs were used as research material, and the RNAi technique was used to knockdown the endogenous WT1. The results show that WT1 knockdown did not affect the proliferation ability of SCs, but down-regulated the expression of polarity-associated proteins (Par3, Par6b, and E-cadherin), junction-associated protein (occludin) and inhibits transcription of downstream genes (WNT4, JNK, αPKC, and CDC42) in non-canonical WNT signaling pathway. WT1 also altered ZO-1 and occludin protein distribution. Overexpression of WNT1 did not affect the expression of Par6b, E-cadherin, and occludin, whereas the non-canonical WNT signaling pathway inhibitors wnt-c59, CCG-1423, and GO-6983 down-regulated the expression of Par6b, E-cadherin, and occludin respectively. This study indicates that WT1 mediates the regulation of several proteins involved in bovine SCs polarity maintenance and intercellular tight junctions (TJ) by non-canonical WNT signaling pathway. |
| Databáze: | OpenAIRE |
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