In-vivo wound healing activity of a novel composite sponge loaded with mucilage and lipoidal matter of Hibiscus species
| Autor: | Asmaa K. Al-Mokaddem, Abd El-Nasser G. El-Gendy, Sameh S. Gad, Dalia A. Attia, Reham I. Amer, Marwa A. A. Fayed, Ahlam M. El-Fishawy, Riham O. Bakr, Mai M. Abdelhafez |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Surgical Sponges Vascular Endothelial Growth Factor A 0301 basic medicine Mucilage Wounds Penetrating RM1-950 Administration Cutaneous Cell Line Chitosan 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Re-Epithelialization Transforming Growth Factor beta Glucosamine Animals Humans Petroleum ether Rats Wistar Skin Pharmacology Wound Healing Chromatography biology Plant Extracts Tumor Necrosis Factor-alpha General Medicine biology.organism_classification Hibiscus Lipids Wound-healing sponge Disease Models Animal Sponge 030104 developmental biology Lipoidal matter chemistry 030220 oncology & carcinogenesis Drug delivery Therapeutics. Pharmacology Inflammation Mediators Wound healing |
| Zdroj: | Biomedicine & Pharmacotherapy, Vol 135, Iss, Pp 111225-(2021) |
| ISSN: | 0753-3322 |
| Popis: | Many researches have been undergone to hasten the natural wound healing process. In this study, several Hibiscus species (leaves) were extracted with petroleum ether, methanol, and their mucilage was separated. All the tested species extracts were assessed for their viability percentage using the water-soluble tetrazolium. H.syriacus was the plant of choice to be incorporated in a new drug delivery system and evaluated for its wound healing activity. H.syriacus petroleum ether extract (PEE) showed a high percentage of palmitic and oleic acids while its mucilage demonstrated high glucosamine and galacturonic acid. It was selected to be formulated and pharmaceutically evaluated into three different composite sponges using chitosan in various ratios. Fourier-transformed infrared spectroscopy investigated the chemical interaction between the utilized sponges' ingredients. Morphological characteristics were evaluated using scanning electron microscopy. H.syriacus composite sponge of mucilage: chitosan (1:5) was loaded with three different concentrations of PEE. Medicated formulations were assessed in rat model of excision wound model. The wound healing ability was clearly proved by the clinical acceleration, histopathological examination, and modulation of correlated inflammatory parameters as tumor necrosis factor in addition to vascular endothelial growth factor suggesting a promising valuable candidate that supports the management of excision wounds using single-dose preparation. |
| Databáze: | OpenAIRE |
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