Immune Profiling Panel: A Proof-of-Concept Study of a New Multiplex Molecular Tool to Assess the Immune Status of Critically Ill Patients

Autor: Julien Textoris, Fabienne Venet, Andrew Hemmert, Virginie Moucadel, Laurence Vachot, Sophie Blein, Dina M. Tawfik, Adeline Bocquet, Thomas Rimmelé, François Mallet, Alexandre Pachot, Javier Yugueros-Marcos, Guillaume Monneret, Montgomery Jesse Linton, Karen Brengel-Pesce
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Oncology
Male
medicine.medical_specialty
in vitro diagnostic
FilmArray
Secondary infection
Critical Illness
critically ill patients
Supplement Articles
Immunologic Tests
Proof of Concept Study
immune response
Monocytes
law.invention
Sepsis
sepsis
03 medical and health sciences
0302 clinical medicine
Immune system
law
Internal medicine
Multiplex polymerase chain reaction
Immunology and Allergy
Medicine
Humans
Multiplex
AcademicSubjects/MED00860
Aged
business.industry
Septic shock
biomarkers
030208 emergency & critical care medicine
HLA-DR Antigens
multiplex PCR
Middle Aged
medicine.disease
Intensive care unit
Shock
Septic
030104 developmental biology
Infectious Diseases
Real-time polymerase chain reaction
AcademicSubjects/MED00290
syndromic panel
Female
business
Multiplex Polymerase Chain Reaction
Zdroj: The Journal of Infectious Diseases
ISSN: 1537-6613
0022-1899
Popis: Background Critical illness such as sepsis is a life-threatening syndrome defined as a dysregulated host response to infection and is characterized by patients exhibiting impaired immune response. In the field of diagnosis, a gap still remains in identifying the immune profile of critically ill patients in the intensive care unit (ICU). Methods A new multiplex immune profiling panel (IPP) prototype was assessed for its ability to semiquantify messenger RNA immune-related markers directly from blood, using the FilmArray System, in less than an hour. Samples from 30 healthy volunteers were used for the technical assessment of the IPP tool. Then the tool was clinically assessed using samples from 10 healthy volunteers and 20 septic shock patients stratified using human leukocyte antigen–DR expression on monocytes (mHLA-DR). Results The IPP prototype consists of 16 biomarkers that target the immune response. The majority of the assays had a linear expression with different RNA inputs and a coefficient of determination (R2) > 0.8. Results from the IPP pouch were comparable to standard quantitative polymerase chain reaction and the assays were within the limits of agreement in Bland–Altman analysis. Quantification cycle values of the target genes were normalized against reference genes and confirmed to account for the different cell count and technical variability. The clinical assessment of the IPP markers demonstrated various gene modulations that could distinctly differentiate 3 profiles: healthy volunteers, intermediate mHLA-DR septic shock patients, and low mHLA-DR septic shock patients. Conclusions The use of IPP showed great potential for the development of a fully automated, rapid, and easy-to-use immune profiling tool. The IPP tool may be used in the future to stratify critically ill patients in the ICU according to their immune status. Such stratification will enable personalized management of patients and guide treatments to avoid secondary infections and lower mortality.
Databáze: OpenAIRE
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