PTPN23 binds the dynein adaptor BICD1 and is required for endocytic sorting of neurotrophin receptors
| Autor: | Ambrosius P. Snijders, Lucy M. Collinson, Matthew Golding, David Villarroel-Campos, Giampietro Schiavo, Anne Weston, Marta I. Budzinska |
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| Rok vydání: | 2019 |
| Předmět: |
Motor neuron
Endosome Dynein Endocytic cycle Tropomyosin receptor kinase B Receptors Nerve Growth Factor ESCRT 03 medical and health sciences Mice p75NTR 0302 clinical medicine Neurotrophic factors Animals Receptor trkB Receptor 030304 developmental biology 0303 health sciences Trafficking biology Brain-Derived Neurotrophic Factor TrkB Dyneins Cell Biology Protein Tyrosine Phosphatases Non-Receptor Phosphoric Monoester Hydrolases Cell biology Protein Transport nervous system biology.protein Intracellular sorting 030217 neurology & neurosurgery Neurotrophin Research Article |
| Zdroj: | Journal of Cell Science |
| ISSN: | 1477-9137 |
| Popis: | Signalling by target-derived neurotrophins is essential for the correct development of the nervous system and its maintenance throughout life. Several aspects concerning the lifecycle of neurotrophins and their receptors have been characterised over the years, including the formation, endocytosis and trafficking of signalling-competent ligand–receptor complexes. However, the molecular mechanisms directing the sorting of activated neurotrophin receptors are still elusive. Previously, our laboratory identified Bicaudal-D1 (BICD1), a dynein motor adaptor, as a key factor for lysosomal degradation of brain-derived neurotrophic factor (BDNF)-activated TrkB (also known as NTRK2) and p75NTR (also known as NGFR) in motor neurons. Here, using a proteomics approach, we identified protein tyrosine phosphatase, non-receptor type 23 (PTPN23), a member of the endosomal sorting complexes required for transport (ESCRT) machinery, in the BICD1 interactome. Molecular mapping revealed that PTPN23 is not a canonical BICD1 cargo; instead, PTPN23 binds the N-terminus of BICD1, which is also essential for the recruitment of cytoplasmic dynein. In line with the BICD1-knockdown phenotype, loss of PTPN23 leads to increased accumulation of BDNF-activated p75NTR and TrkB in swollen vacuole-like compartments, suggesting that neuronal PTPN23 is a novel regulator of the endocytic sorting of neurotrophin receptors. Highlighted Article: PTPN23 associates with BICD1 via its dynein-binding region, and its loss results in accumulation of neurotrophin receptors in enlarged, ubiquitylated sorting compartments. |
| Databáze: | OpenAIRE |
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