Lingguizhugan decoction attenuates diet-induced obesity and hepatosteatosisviagut microbiota
| Autor: | Ting-Ying Zhang, Meng-Ting Liu, Ying-Juan Huang, Jian Qin, Xifeng Lu, Lun-Bo Tan |
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| Rok vydání: | 2019 |
| Předmět: |
Male
medicine.medical_specialty Decoction Gut microbiota Gut flora Diet High-Fat digestive system Mice 03 medical and health sciences fluids and secretions 0302 clinical medicine Non-alcoholic Fatty Liver Disease Internal medicine Diabetes mellitus medicine Animals Humans Obesity Intestinal Mucosa Caloric Restriction biology Plant Extracts business.industry Diabetes digestive oral and skin physiology Gastroenterology Hepatosteatosis Lipid metabolism General Medicine Basic Study Lipid Metabolism medicine.disease biology.organism_classification Combined Modality Therapy Lipids Gastrointestinal Microbiome Mice Inbred C57BL Disease Models Animal stomatognathic diseases Treatment Outcome Endocrinology Liver 030220 oncology & carcinogenesis 030211 gastroenterology & hepatology business |
| Zdroj: | World Journal of Gastroenterology |
| ISSN: | 1007-9327 |
| Popis: | BACKGROUND Obesity is a major risk factor for a variety of diseases such as diabetes, nonalcoholic fatty liver disease, and cardiovascular diseases. Restricting energy intake, or caloric restriction (CR), can reduce body weight and improve metabolic parameters in overweight or obese patients. We previously found that Lingguizhugan decoction (LZD) in combination with CR can effectively lower plasma lipid levels in patients with metabolic syndrome. However, the mechanism underlying CR and LZD treatment is still unclear. AIM To investigate whether CR and LZD improve metabolic parameters by modulating gut microbiota. METHODS We extracted the water-soluble components out of raw materials and dried as LZD extracts. Eight-week old male C57BL/6 mice were treated with a 3-d treatment regime that included 24 h-fasting followed by gavage of LZD extracts for 2 consecutive days, followed by a normal diet (ND) ad libitum for 16 wk. To test the effects of gut microbiota on diet-induced obesity, 8-wk old male C57BL/6 mice received fecal microbiota transplantation (FMT) from CR and LZD-treated mice every 3 d and were fed with high-fat diet (HFD) ad libitum for 16 wk. Control mice received either saline gavage or FMT from ND-fed mice receiving saline gavage as mentioned above. Body weight was monitored bi-weekly. Food consumption of each cage hosting five mice was recorded weekly. To monitor blood glucose, total cholesterol, and total triglycerides, blood samples were collected via submandibular bleeding after 6 h fasting. Oxygen consumption rate was monitored with metabolic cages. Feces were collected, and fecal DNA was extracted. Profiles of gut microbiota were mapped by metagenomic sequencing. RESULTS We found that CR and LZD treatment significantly reduced the body weight of mice fed with ND (28.71 ± 0.29 vs 28.05 ± 0.15, P < 0.05), but did not affect plasma total cholesterol or total triglyceride levels. We then transplanted the fecal microbiota collected from CR and LZD-treated mice under ND feeding to HFD-fed mice. Intriguingly, transplanting the mice with fecal microbiota from CR and LZD-treated mice potently reduced body weight (44.95 ± 1.02 vs 40.53 ± 0.97, P < 0.001). FMT also reduced HFD-induced hepatosteatosis, in addition to improved glycemic control. Mechanistic studies found that FMT increased OCR of the mice and suppressed the expression and protein abundance of lipogenic genes in the liver. Metagenomic analysis revealed that HFD drastically altered the profile of gut microbiota, and FMT modified the profile of the gut microbiota. CONCLUSION Our study suggests that CR and LZD improve metabolic parameters by modulating gut microbiota. |
| Databáze: | OpenAIRE |
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