Increased fatty acid synthesis inhibits nitrogen starvation-induced autophagy in lipid droplet-deficient yeast
| Autor: | Pierre Voisin, Yves Y. Sere, François-Xavier Bernard, Christine Barrault, Venty M.S. Soeroso, Bin Wan, Thierry Bergès, Marianne Bernard, Matthieu Régnacq, Nadine Camougrand |
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| Přispěvatelé: | Signalisation et Transports Ioniques Membranaires (STIM), Université de Poitiers-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut de biochimie et génétique cellulaires (IBGC), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), BIOALTERNATIVES, Université de Poitiers-Université de Tours-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Autophagosome Nitrogen Biophysics Saccharomyces cerevisiae Fatty acid synthase Biochemistry 03 medical and health sciences chemistry.chemical_compound Lipid droplet Organelle Autophagy [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Rapamycin Molecular Biology Fatty acid synthesis biology Fatty Acids Lipid metabolism Cell Biology Lipid Metabolism Fatty acid Lipid droplets Cerulenin 030104 developmental biology chemistry biology.protein lipids (amino acids peptides and proteins) |
| Zdroj: | Biochemical and Biophysical Research Communications Biochemical and Biophysical Research Communications, Elsevier, 2016, 477 (1), pp.33-39. ⟨10.1016/j.bbrc.2016.06.017⟩ |
| ISSN: | 0006-291X 1090-2104 |
| Popis: | Biologie végétale; International audience; Macroautophagy is a degradative pathway whereby cells encapsulate and degrade cytoplasmic material within endogenously-built membranes. Previous studies have suggested that autophagosome membranes originate from lipid droplets. However, it was recently shown that rapamycin could induce autophagy in cells lacking these organelles. Here we show that lipid droplet-deprived cells are unable to perform autophagy in response to nitrogen-starvation because of an accelerated lipid synthesis that is not observed with rapamycin. Using cerulenin, a potent inhibitor of fatty acid synthase, and exogenous addition of palmitic acid we could restore nitrogen-starvation induced autophagy in the absence of lipid droplets. |
| Databáze: | OpenAIRE |
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