Circulatory miRNA biomarkers of metabolic syndrome
| Autor: | V Miranda-Soberanis, David Cameron-Smith, Sally D. Poppitt, Brenan R. Durainayagam, Randall F. D'Souza, James F. Markworth, Nicole C. Roy, Farha Ramzan, Ivana R. Sequeira, Amber M. Milan, Cameron J. Mitchell |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
Male Endocrinology Diabetes and Metabolism medicine.medical_treatment In silico 030209 endocrinology & metabolism 030204 cardiovascular system & hematology Bioinformatics Real-Time Polymerase Chain Reaction Pathogenesis Cohort Studies 03 medical and health sciences 0302 clinical medicine Endocrinology Insulin resistance Diabetes mellitus microRNA Internal Medicine medicine Humans Metabolic Syndrome business.industry Insulin Wnt signaling pathway General Medicine Middle Aged medicine.disease MicroRNAs Female Metabolic syndrome business Biomarkers |
| Zdroj: | Acta diabetologica. 57(2) |
| ISSN: | 1432-5233 |
| Popis: | Circulatory microRNAs (c-miRNAs) exert important roles in the molecular dysregulation of cardio-metabolic diseases. However, little is known whether dysregulated miRNA expression occurs when risk factors are elevated, as in the metabolic syndrome (MetS). This study quantified c-miRNA expression in individuals with MetS compared to healthy, further examining the relationship of gene pathways with the underlying pathogenesis. Expression of 26 miRNAs was quantified in plasma from 40 women (20 healthy and 20 MetS) and 39 men (20 healthy and 19 MetS) by qPCR. In silico analysis was performed to investigate biological effects of the dysregulated miRNAs. Dysregulated miRNA expression was further validated in an independent cohort of 20 women (10 healthy and 10 MetS). Regression model adjusted for age and sex identified miR-15a-5p, miR-17-5p, miR-370-3p and miR-375 as important predictors of MetS presence. Analysis of predictive miRNAs in the validation cohort strengthened the relationship with miR-15a-5p and miR-17-5p expression. These miRNAs share genes involved in the regulation of metabolic pathways including insulin, wnt, fatty acid metabolism and AMPK. miR-15a-5p and miR-17-5p were identified as predictive biomarkers of MetS, irrespective of sexes, further demonstrating the relationship of c-miRNAs to known pathways of metabolic disturbances present in cardio-metabolic diseases. |
| Databáze: | OpenAIRE |
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