Biosynthesis of biopterin in Ascaris lumbricoides suum
| Autor: | Hidetsugu Otsuka, Miki Goto, Katsura Sugiura |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Sepiapterin
GTP' Biophysics Biopterin Biochemistry Chromatography DEAE-Cellulose chemistry.chemical_compound Column chromatography Multienzyme Complexes Animals Molecular Biology chemistry.chemical_classification biology Ascaris Muscles Pteridines biology.organism_classification Body Fluids Pterins Alcohol Oxidoreductases Enzyme chemistry Sephadex Chromatography Gel Female Guanosine Triphosphate Ascaris lumbricoides |
| Zdroj: | Biochimica et biophysica acta. 629(1) |
| ISSN: | 0006-3002 |
| Popis: | In in vivo experiments, radioactivity from [U-14C]GTP was incorporated into biopterin, and, in fact, all carbon atoms of biopterin synthesized in Ascaris lu lumbricoides suum originated from GTP. Biopterin was also biosynthesized in homogenates of tissue fluid and muscles of Ascaris lumbricoides suum. The enzyme which catalyzes sepiapterin synthesis from D-erythto-7,8-dihydroneopterin-3′-phosphate was found in A. lumbricoides suum extracts and extracted in the 0–30% (NH4)2SO4 fraction from a 40 000 × g supernatant. The enzyme was purified by Sephadex G-200 column and DEAE-cellulose column chromatography. It is suggested that sepiapterin could be an intermediate compound in biopterin biosynthesis. |
| Databáze: | OpenAIRE |
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