Analysis of Discrimination Techniques for Low-Cost Narrow-Band Spectrofluorometers
Autor: | Sergio Pérez, Albert-Miquel Sánchez, Jaume Piera, Ismael F. Aymerich |
---|---|
Rok vydání: | 2015 |
Předmět: |
Normalization (statistics)
Signal processing Engineering Time Factors 010504 meteorology & atmospheric sciences Noise reduction Wavelet Analysis lcsh:Chemical technology computer.software_genre 01 natural sciences Biochemistry Article Fluorescence Analytical Chemistry Transformation Cassification Wavelet lcsh:TP1-1185 14. Life underwater Sensitivity (control systems) Electrical and Electronic Engineering Low-cost sensors Instrumentation 0105 earth and related environmental sciences Block (data storage) Principal Component Analysis Denoising business.industry Taxonomic discrimination 010401 analytical chemistry Process (computing) Signal Processing Computer-Assisted Reference Standards Atomic and Molecular Physics and Optics 0104 chemical sciences Normalization Spectrometry Fluorescence Transformation (function) classification Costs and Cost Analysis Data mining business computer Algorithms |
Zdroj: | Sensors Volume 15 Issue 1 Pages 611-634 Sensors, Vol 15, Iss 1, Pp 611-634 (2014) Sensors (Basel, Switzerland) Digital.CSIC. Repositorio Institucional del CSIC instname |
ISSN: | 1424-8220 2011-3048 |
Popis: | 24 pages, 12 figures, 12 tables The need for covering large areas in oceanographic measurement campaigns and the general interest in reducing the observational costs open the necessity to develop new strategies towards this objective, fundamental to deal with current and future research projects. In this respect, the development of low-cost instruments becomes a key factor, but optimal signal-processing techniques must be used to balance their measurements with those obtained from accurate but expensive instruments. In this paper, a complete signal-processing chain to process the fluorescence spectra of marine organisms for taxonomic discrimination is proposed. It has been designed to deal with noisy, narrow-band and low-resolution data obtained from low-cost sensors or instruments and to optimize its computational cost, and it consists of four separated blocks that denoise, normalize, transform and classify the samples. For each block, several techniques are tested and compared to find the best combination that optimizes the classification of the samples. The signal processing has been focused on the Chlorophyll-a fluorescence peak, since it presents the highest emission levels and it can be measured with sensors presenting poor sensitivity and signal-to-noise ratios. The whole methodology has been successfully validated by means of the fluorescence spectra emitted by five different cultures. © 2014 by the authors; licensee MDPI, Basel, Switzerland This work was supported by the Spanish National Research Council (CSIC) under the project ANERIS (PIF-015-1), by the Ministerio de Ciencia e Innovación under Mestral Project CTM2011-30489-C02-01, and by the European Commission under Citclops Project FP7-ENV-308469. Ismael F. Aymerich is currently funded by the European Regional Development Fund (ERDF) and the Galician Regional Government under agreement for funding the Atlantic Research Center for Information and Communication Technologies (AtlantTIC) and under the project TACTICA. Sergio Pérez was involved in the SICUE program and funded by the Séneca fellowship, given by the Ministerio de Educación, during the development of this project |
Databáze: | OpenAIRE |
Externí odkaz: |