Listeria monocytogenes induced dysbiosis in snails and rebiosis achieved by administration of the gut commensal Lactobacillus plantarum Sgs14 strain
| Autor: | Esmeralda Dushku, Kalodoti Avgousti, Antonios Zdragas, Charalampos Kotzamanidis, Minas Yiangou, George Vafeas, Virginia Giantzi, Alexandra Staikou |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Snails Snail Aquatic Science medicine.disease_cause law.invention Microbiology 03 medical and health sciences Probiotic Immune system Listeria monocytogenes law biology.animal Hemolymph medicine Environmental Chemistry Animals Listeriosis biology Probiotics 04 agricultural and veterinary sciences General Medicine medicine.disease biology.organism_classification Mucus Intestinal epithelium Gastrointestinal Microbiome 030104 developmental biology 040102 fisheries 0401 agriculture forestry and fisheries Dysbiosis Lactobacillus plantarum |
| Zdroj: | Fishshellfish immunology. 104 |
| ISSN: | 1095-9947 |
| Popis: | Listeria monocytogenes strains were isolated from Cornu aspersum maxima snails from farm units experiencing high mortalities and were characterized by phenotypic, molecular and biochemical criteria. A high heterogeneity was observed in the pulsed-field gel electrophoresis (PFGE) pulsotypes as well as in the virulence (13-100% mortality) among the fifteen L. monocytogenes strains. One strain was characterized as non-virulent while three strains exhibited hypervirulent phenotype. Hypervirulence activity was associated with cell surface properties such as hydrophobicity, autoaggregation and biofilm formation, with increased tolerance to snail's gut barriers such as pedal mucus, gastric mucus, gastric juices, and acidic pH as well as with increased capacity to resist the antibacterial activity of snail haemolymph and modulate immune cell populations and functions such as chemotaxis and phagocytoses. L. monocytogenes dysbiosis was characterized by a clinicopathological phenotype including immobilization of snails' headfoot outside the shell, increased mucus-secreting cells in the intestinal epithelium and feces, alteration of intestinal ridges morphology and excessive increase of haemolymph immune cells and cell death. Rebiosis in L. monocytogenes SN3 strain infected snails was achieved by dietary supplementation of the snail-gut commensal probiotic L. plantarum Sgs14 strain by exhibiting anti-Listeria activity, reducing mortality and clinicopathological manifestations as well as exhibiting immunomodulatory activity. |
| Databáze: | OpenAIRE |
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