Contribution of ATP synthase to stimulation of respiration by Ca2+ in heart mitochondria
| Autor: | G. Baliutyte, Vida Mildaziene, Rasa Baniene, S. Maslauskaite, Zita Nauciene |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Metabolic Clearance Rate Cell Respiration Guinea Pigs Statistics as Topic Succinic Acid Stimulation Mitochondrion Mitochondria Heart Guinea pig Species Specificity Respiration Genetics Animals Computer Simulation Calcium Signaling Rats Wistar Respiratory system Molecular Biology Cells Cultured ATP synthase biology Models Cardiovascular Cell Biology Mitochondrial Proton-Translocating ATPases Rats Enzyme Activation Biochemistry Modeling and Simulation biology.protein Molecular Medicine Calcium Rabbits Respiration rate Flux (metabolism) Biotechnology |
| Zdroj: | IEE Proceedings - Systems Biology. 153:350 |
| ISSN: | 1741-2471 |
| Popis: | A variety of experimental conditions were applied with the aim to estimate the correlation between the contribution of ATP synthase to the respiratory flux control and the calcium-induced activation of succinate oxidation in heart mitochondria isolated from rat, rabbit and guinea pig. The sensitivity of respiration in heart mitochondria to the decrease in temperature from 37 degrees C to 28 degrees C decreases in the order rabbit > guinea pig > rat. Ca2+ effect on succinate oxidation rate in state 3 respiration was species- and temperature-dependent and ranged from 0 (rat, 37 degrees C) to +44% (rabbit, 28 degrees C). For mitochondria from all experimental animals, the increase of Ca2+ in physiological range of concentration did not change state 2 respiration rate, and the stimulatory effect of Ca2+ on state 3 respiration was more pronounced at 28 degrees C than at 37 degrees C. The respiratory subsystem was sensitive to Ca2+ ions only in rabbit heart mitochondria. A high positive correlation between Ca2+ ability to stimulate succinate oxidation in state 3 and the control exerted by ATP synthase over the respiratory flux provides argument confirming stimulation of ATP synthase by Ca2+ ions. |
| Databáze: | OpenAIRE |
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