Kinetic study of glomerular cells of diabetic rats in relation to the development of irreversible glomerular sclerosis
| Autor: | Gengo Osawa, Yuichi Kitano, Tamaki Sasaki, Masahiko Yamada, Tetsuya Sato |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
medicine.medical_specialty
medicine.drug_class Endocrinology Diabetes and Metabolism Kidney Glomerulus Labeling index Monoclonal antibody Diabetes Mellitus Experimental Diabetic nephropathy chemistry.chemical_compound Endocrinology Internal medicine DNA precursor Internal Medicine medicine Animals Diabetic Nephropathies Mitosis business.industry Glomerulosclerosis Rats Inbred Strains medicine.disease Capillaries Glomerular Mesangium Rats Cellular kinetics Kinetics Microscopy Electron chemistry business Bromodeoxyuridine |
| Zdroj: | Journal of Diabetic Complications. 5:115-117 |
| ISSN: | 0891-6632 |
| DOI: | 10.1016/0891-6632(91)90038-q |
| Popis: | In order to clarify the cellular kinetics involved in progression of diabetic glomerulosclerosis, the epithelial cells of spontaneously diabetic WBNKob rats were investigated by labeling with the DNA precursor bromodeoxyuridine, which was histochemically stained using monoclonal antibody. The labeling index (number of labeled cells/number of cells counted) of glomeruli was constantly low (approximately 0.15%) and no significant variation could be demonstrated in control rats of the Wistar strain. In both control and diabetic rats, podocytes showed no evidence of mitosis. In contrast to capillary podocytes, the Bowman epithelial cells of diabetic rats showed increased labeling, often surrounding sclerotic capillaries. These observations suggest a critical role of Bowman's epithelial cells in the development of irreversibly sclerotic glomeruli in diabetic nephropathy. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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