High density lipoproteins stimulate the production and secretion of endothelin-1 from cultured bovine aortic endothelial cells
| Autor: | Mei-Yu Chuang, Ali Pedram, Moti L. Kashyap, Harrison J.L. Frank, Ellis R. Levin, Bruce A. Prins, Ren-Ming Hu |
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| Rok vydání: | 1994 |
| Předmět: |
medicine.medical_specialty
Vascular smooth muscle Endothelium Stimulation Biology chemistry.chemical_compound Internal medicine medicine Animals Secretion RNA Messenger Aorta Cells Cultured Protein Kinase C Cholesterol Endothelins nutritional and metabolic diseases General Medicine Endothelin 1 Endothelial stem cell Endocrinology medicine.anatomical_structure chemistry Cattle lipids (amino acids peptides and proteins) Endothelium Vascular Lipoproteins HDL Endothelin secretion Research Article |
| Zdroj: | Journal of Clinical Investigation. 93:1056-1062 |
| ISSN: | 0021-9738 |
| DOI: | 10.1172/jci117055 |
| Popis: | The concentration of HDL in the blood inversely correlates with the incidence of cardiovascular disease, probably related to the ability of these lipoproteins to efflux cholesterol from vascular cells. it is also possible that HDL affect the production or action of vasoactive peptides implicated in the development of vascular diseases. Therefore, we determined the effects of human HDL on the production and secretion of endothelin-1 (ET-1) from cultured bovine aortic endothelial cells. HDL produced a highly significant stimulation of endothelin secretion (maximum 240% of control), even at very low levels of lipoproteins (1 microgram/ml). HDL also stimulated the translation of ET-1 by twofold in the bovine aortic endothelial cells. In contrast, HDL had no significant effect on steady state mRNA levels, transcript degradation, or transcription. Stimulation of ET-1 secretion by HDL was dependent on protein kinase C activation. Purified apo A-I, the major apoprotein of HDL, increased ET-1 secretion and translation approximately 85% as potently as HDL. Our results indicate that low concentrations of human HDL strongly stimulate the production of ET-1, a powerful vasoconstrictor and mitogen for the vascular smooth muscle cell. We propose that HDL may participate in the regulation of vasomotor tone through this potentially important effect in the vasculature. |
| Databáze: | OpenAIRE |
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