Involvement of AmpG in mediating a dynamic relationship between serine beta-lactamase induction and biofilm-forming ability of Escherichia coli
| Autor: | Anindya S. Ghosh, Shilpa Pal, Dhriti Mallik |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Lysis medicine.medical_treatment 030106 microbiology Microbial Sensitivity Tests medicine.disease_cause beta-Lactams Microbiology beta-Lactamases Serine 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins Molecular genetics Genetics medicine Escherichia coli Molecular Biology Chemistry Permease Biofilm Membrane Transport Proteins biochemical phenomena metabolism and nutrition Anti-Bacterial Agents 030104 developmental biology Biofilms Beta-lactamase Peptidoglycan |
| Zdroj: | FEMS microbiology letters. 365(8) |
| ISSN: | 1574-6968 |
| Popis: | AmpG permease is implicated both in beta-lactamase induction and peptidoglycan recycling in enterobacterial isolates. Here, physiological studies using molecular genetics show that deletion of AmpG permease dramatically increases beta-lactam susceptibility even in the presence of AmpC, TEM-1 and OXA beta-lactamases. Also, there is an appreciable decrease in the biofilm-forming ability of strains lacking this protein. Expression of this permease in excess probably compromises the integrity of the bacterial cells, leading to cell lysis. Based on these results, we propose that AmpG permease may be used as a potential antibiotic target and its suppression could efficiently inhibit both beta-lactamase induction and biofilm formation. |
| Databáze: | OpenAIRE |
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