The Use of Human Sterilized Crushed Tooth Particles Compared with BTCP Biomaterial and Empty Defects in Bone Formation inside Critical Rabbit Calvaria Sites

Autor: José Luis Calvo-Guirado, Marta Belén Cabo-Pastor, Francisco Martínez-Martínez, Miguel Ángel Garcés-Villalá, Félix de Carlos-Villafranca, Nuria García-Carrillo, Manuel Fernández-Domínguez
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Bioengineering; Volume 10; Issue 6; Pages: 638
ISSN: 2306-5354
DOI: 10.3390/bioengineering10060638
Popis: This study aimed to assess the bone regeneration of critical-size defects in rabbit calvaria filled with freshly crushed extracted teeth, comparing them with BTCP biomaterial and empty sites. Materials and methods: Twenty-one female New Zealand rabbits were used in this study. Two critical-size defects 6 mm in size were created in the skull bone, each with a 3 mm separation between them. Three experimental groups were evaluated: Group A (human sterilized crushed teeth granules alone), Group B (Bioner Bone, Bioner Sitemas Implantológicos), and Group C (unfilled defects). The animals were sacrificed at 4 and 8 weeks. Evaluation of the samples involved histological and histomorphometric analyses with radiographic evaluation. The histological evaluation showed a higher volume reduction in Group A compared with Group B (p < 0.05) and Control. Group A showed the highest values for cortical closure and bone formation around the particles, followed by Group B and Group C (p < 0.05). Within the limitations of this animal study, we can conclude that the use of human tooth particles leads to increased bone formation and reduced connective tissue in critical-size defects in rabbit calvaria when compared to BTCP biomaterial. The calvarial model is a robust base for the evaluation of different biomaterials.
Databáze: OpenAIRE
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