Developed and validated stability indicating HPLC method for the determination of epirubicin in bulk drug, marketed injection and polymeric nanoparticles
| Autor: | Anu T. Singh, Shindu C. Thomas, Mohammad Tariq, Sushama Talegaonkar |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
lcsh:RS1-441
030226 pharmacology & pharmacy 01 natural sciences High-performance liquid chromatography Dosage form Polymeric nanoparticles lcsh:Pharmacy and materia medica 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Acetonitrile Epirubicin Detection limit Chromatography 010401 analytical chemistry Quality control Repeatability Forced degradation 0104 chemical sciences chemistry Methanol HPLC medicine.drug |
| Zdroj: | Brazilian Journal of Pharmaceutical Sciences, Volume: 54, Issue: 4, Article number: e17515, Published: 08 APR 2019 Brazilian Journal of Pharmaceutical Sciences; Vol. 54 No. 4 (2018); e17515 Brazilian Journal of Pharmaceutical Sciences; Vol. 54 Núm. 4 (2018); e17515 Brazilian Journal of Pharmaceutical Sciences; v. 54 n. 4 (2018); e17515 Brazilian Journal of Pharmaceutical Sciences Universidade de São Paulo (USP) instacron:USP Brazilian Journal of Pharmaceutical Sciences, Vol 54, Iss 4 (2019) |
| ISSN: | 2175-9790 1984-8250 |
| Popis: | Present work is aimed to develop a simple, sensitive, robust and reliable HPLC method for routine quality control of epirubicin (EPI) in bulk drug, marketed injections and polymeric nanoparticles. Separation was carried out by C18 column. Isocratic elution was carried out using mobile phase A: 0.16% o-phosphoric acid solution, B: acetonitrile and methanol mixture (80:20, v/v) in the ratio of 60:40 (A: B) while the flow rate was maintained at 1mL/min. Analyses were performed at 233.5 nm using PDA detector. Excellent linear relationship was observed between peak-area versus drug concentration in the range of 1.0‑100.0 μg/mL (r2 , 0.999). Developed method was found to be sensitive (Limits of detection and quantification were found to be ~8 ng/mL and ~25 ng/mL, respectively), precise (RSD |
| Databáze: | OpenAIRE |
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