Molecular characterization and crystallization of Diocleinae lectins
| Autor: | Hubert Thole, Juan J. Calvete, Thalles B. Grangeiro, Fernanda N. Guimarães, Márcio V. Ramos, Benildo Sousa Cavada, Antonio A. Romero, Claus Urbanke, Iza M. A. Rocha, Manfred Raida |
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| Rok vydání: | 1999 |
| Předmět: |
Sequence analysis
Molecular Sequence Data Biophysics Mass spectrometry Biochemistry Mass Spectrometry law.invention Analytical Ultracentrifugation Structure-Activity Relationship X-Ray Diffraction Structural Biology law Lectins Amino Acid Sequence Dioclea guianensis Crystallization Molecular Biology Peptide sequence Plants Medicinal biology Chemistry Protein primary structure Lectin Fabaceae Hydrogen-Ion Concentration biology.organism_classification Molecular Weight Seeds biology.protein Plant Lectins Sequence Alignment |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1430:367-375 |
| ISSN: | 0167-4838 |
| DOI: | 10.1016/s0167-4838(99)00020-5 |
| Popis: | Molecular characterization of seven Diocleinae lectins was assessed by sequence analysis, determination of molecular masses by mass spectrometry, and analytical ultracentrifugation equilibrium sedimentation. The lectins show distinct pH-dependent dimer-tetramer equilibria, which we hypothesize are due to small primary structure differences at key positions. Lectins from Dioclea guianensis, Dioclea virgata, and Cratylia floribunda seeds have been crystallized and preliminary X-ray diffraction analyses are reported. |
| Databáze: | OpenAIRE |
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