Biofunctionalization of titanium surfaces with alendronate and albumin modulates osteoblast performance
| Autor: | Paulo Noronha Lisboa-Filho, Anderson Moreira Gomes, Geórgia da Silva Feltran, Carolina Simão Albano, Willian Fernando Zambuzzi, Luciana D. Trino, Célio Junior da Costa Fernandes |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp) |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cell biology Biocompatibility Article 03 medical and health sciences 0302 clinical medicine In vivo Osseointegration Extracellular medicine Bisphosphonate Zymography Implants lcsh:Social sciences (General) lcsh:Science (General) Cell adhesion Trace elements Multidisciplinary Chemistry Albumin Osteoblast Materials science RUNX2 030104 developmental biology medicine.anatomical_structure lcsh:H1-99 030217 neurology & neurosurgery lcsh:Q1-390 |
| Zdroj: | Heliyon Heliyon, Vol 6, Iss 7, Pp e04455-(2020) Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 2405-8440 |
| Popis: | Background Biofunctionalization of titanium surfaces can improve host responses, especially considering the time for osteointegration and patient recovery. This prompted us to modify titanium surfaces with alendronate and albumin and to investigate the behavior of osteoblasts on these surfaces. Methods The biofunctionalization of titanium surfaces was characterized using classical physicochemical approaches and later used to challenge pre-osteoblast cells up to 24 h. Then their viability and molecular behavior were investigated using mitochondrial dehydrogenase activity and RTq-PCR technologies, respectively. Potential stimulus of extracellular remodeling was also investigated by zymography. Results Our data indicates a differential behavior of cells responding to the surfaces, considering the activity of mitochondrial dehydrogenases. Molecularly, the differential expression of genes related with cell adhesion highlighted the importance of Integrin-β1, Fak, and Src. These 3 genes were significantly decreased in response to titanium surfaces modified with alendronate, but this behavior was reverted when alendronate was associated with albumin. Alendronate-modified surfaces promoted a significant increase on ECM remodeling, as well as culminating with greater gene activity related to the osteogenic phenotype (Runx2, Alp, Bsp). Conclusion Altogether, our study found interesting osteogenic behavior of cells in response to alendronate and albumin surfaces, which indicates the need for in vivo analyses to better consider these surfaces before clinical trials within the biomedical field. Materials science; Cell biology; Implants; Biocompatibility; Trace elements; Osseointegration; Bisphosphonate; Albumin. |
| Databáze: | OpenAIRE |
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