Intradermal DNA immunization: Antisera specific for the membrane lectin MR60/ERGIC-53
| Autor: | Michel Monsigny, Valérie Altemayer, Violaine Carrière, Annie-Claude Roche, Geneviève Motta, Ludovic Landemarre |
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| Předmět: |
Cell Membrane Permeability
Injections Intradermal Biophysics Biochemistry Mice symbols.namesake Antibody Specificity C-type lectin Lectins Animals Humans Molecular Biology Mannan-binding lectin Mice Inbred BALB C biology Immune Sera Endoplasmic reticulum CD69 Membrane Proteins Lectin Cell Biology Golgi apparatus Precipitin Tests Recombinant Proteins Transmembrane protein Rats Cytosol Mannose-Binding Lectins Microscopy Fluorescence COS Cells Mutation biology.protein symbols Female Immunization Plasmids |
| Zdroj: | Scopus-Elsevier |
| Popis: | The trafficking of intracellular membrane proteins in Golgi apparatus, endoplasmic reticulum or intermediate compartment has not yet been fully elucidated. The human MR60/ERGIC-53 and the rat p58 proteins are one such protein; and to study them in cell-free and in situ systems, high quality monospecific antisera are required. Highly specific antisera have been obtained after immunization of mice with plasmids containing a gene encoding either the full length or a truncated protein. The best results were obtained after intradermal injections of a plasmid encoding a truncated protein comprising both the luminal carbohydrate recognition domain and the stem down to a cysteine residue close to the C-terminal end, but neither the transmembrane nor the cytosolic domains. Such antisera have a very high titer and are very efficient tools to visualize the MR60 protein in situ or to selectively precipitate the MR60 proteins from a whole cell lysate. |
| Databáze: | OpenAIRE |
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