The Impact of Lipoprotein-Associated Oxidative Stress on Cell-Specific Microvesicle Release in Patients with Familial Hypercholesterolemia
| Autor: | S. Vedel, Bent Raungaard, Henning Beck-Nielsen, Aase Handberg, Morten Hjuler Nielsen, H. Irvine |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
CD36 Antigens Male Aging medicine.medical_specialty Article Subject CD36 Lipoproteins Familial hypercholesterolemia 030204 cardiovascular system & hematology Biochemistry Achilles Tendon Flow cytometry Hyperlipoproteinemia Type II 03 medical and health sciences 0302 clinical medicine Cell-Derived Microparticles Internal medicine medicine Xanthomatosis Humans Platelet lcsh:QH573-671 biology medicine.diagnostic_test lcsh:Cytology business.industry Microvesicle Cell Biology General Medicine Middle Aged medicine.disease Flow Cytometry Microvesicles Lipoproteins LDL Oxidative Stress 030104 developmental biology Endocrinology biology.protein Regression Analysis Female business Cell activation Lipoprotein Research Article |
| Zdroj: | Nielsen, M H, Irvine, H, Vedel, S, Raungaard, B, Beck-Nielsen, H & Handberg, A 2016, ' The Impact of Lipoprotein-Associated Oxidative Stress on Cell-Specific Microvesicle Release in Patients with Familial Hypercholesterolemia ', Oxidative Medicine and Cellular Longevity, vol. 2016, 2492858 . https://doi.org/10.1155/2016/2492858 Oxidative Medicine and Cellular Longevity Oxidative Medicine and Cellular Longevity, Vol 2016 (2016) |
| DOI: | 10.1155/2016/2492858 |
| Popis: | Objective. Microvesicles (MVs) are small cell-derived particles shed upon activation. Familial hypercholesterolemia (FH) particularly when associated with Achilles tendon xanthomas (ATX) predisposes to atherosclerosis, possibly through oxLDL-C interaction with the CD36 receptor. To investigate the hypothesis that MVs derived from cells involved in atherosclerosis are increased in FH and that CD36 expressing MVs (CD36+ MVs) may be markers of oxLDL-C-induced cell activation, cell-specific MVs were measured in FH patients with and without ATX and their association with atherogenic lipid profile was studied.Approach and Results. Thirty FH patients with and without ATX and twenty-three controls were included. Plasma concentrations of MVs and CD36+ MVs derived from platelets (PMVs), erythrocytes (ErytMVs), monocytes (MMVs), and endothelial cells (EMVs), as well as tissue factor-positive cells (TF+ MVs), were measured by flow cytometry. Total MVs, MMVs, EMVs, ErytMVs, and TF+ MVs were significantly increased in FH patients, compared to controls. CD36+ MVs derived from endothelial cells and monocytes were significantly higher in FH patients and oxLDL-C predicted all the investigated cell-specific CD36+ MVs in FH patients with ATX.Conclusions. MVs derived from cells involved in atherosclerosis were increased in FH and may contribute to elevated atherothrombosis risk. The increased cell-specific CD36+ MVs observed in FH may represent markers of oxLDL-C-induced cell activation. |
| Databáze: | OpenAIRE |
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