Telomere Length in Chromosomally Normal and Abnormal Miscarriages and Ongoing Pregnancies and Its Association with 5-hydroxymethylcytosine Patterns

Autor: Mikhail I. Krapivin, Olga E. Talantova, Vera S. Dudkina, Olga A. Efimova, Andrei V. Tikhonov, Anna A. Pendina, Anna A Smirnova, Vladislav S Baranov, Olga G. Chiryaeva, Lubov’ I. Petrova
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
miscarriage
Miscarriage
0302 clinical medicine
telomere length
Lymphocytes
Biology (General)
Spectroscopy
reproductive and urinary physiology
Karyotype
Embryo
Chorion
General Medicine
Telomere
Trophoblasts
Computer Science Applications
Chemistry
medicine.anatomical_structure
030220 oncology & carcinogenesis
embryonic structures
5-Methylcytosine
Female
pregnancy
heredity
QH301-705.5
Biology
Article
Catalysis
Inorganic Chemistry
Andrology
03 medical and health sciences
medicine
Humans
Sister chromatids
5-hydroxymethylcytosine
Physical and Theoretical Chemistry
Molecular Biology
Metaphase
QD1-999
Pregnancy
Cytotrophoblast
epigenetics
Organic Chemistry
Telomere Homeostasis
DNA Methylation
medicine.disease
telomeres
Abortion
Spontaneous
Pregnancy Trimester
First
030104 developmental biology
Case-Control Studies
chorionic cytotrophoblast
Zdroj: International Journal of Molecular Sciences, Vol 22, Iss 6622, p 6622 (2021)
International Journal of Molecular Sciences
Volume 22
Issue 12
ISSN: 1661-6596
1422-0067
Popis: The present study investigates telomere length (TL) in dividing chorionic cytotrophoblast cells from karyotypically normal and abnormal first trimester miscarriages and ongoing pregnancies. Using Q-FISH, we measured relative TLs in the metaphase chromosomes of 61 chorionic villous samples. Relative TLs did not differ between karyotypically normal samples from miscarriages and those from ongoing pregnancies (p = 0.3739). However, among the karyotypically abnormal samples, relative TLs were significantly higher in ongoing pregnancies than in miscarriages (p <
0.0001). Relative TLs were also significantly higher in chorion samples from karyotypically abnormal ongoing pregnancies than in those from karyotypically normal ones (p = 0.0018) in contrast to miscarriages, where relative TL values were higher in the karyotypically normal samples (p = 0.002). In the karyotypically abnormal chorionic cytotrophoblast, the TL variance was significantly lower than in any other group (p <
0.05). Assessed by TL ratios between sister chromatids, interchromatid TL asymmetry demonstrated similar patterns across all of the chorion samples (p = 0.22) but significantly exceeded that in PHA-stimulated lymphocytes (p <
0.0001, p = 0.0003). The longer telomere was predominantly present in the hydroxymethylated sister chromatid in chromosomes featuring hemihydroxymethylation (containing 5-hydroxymethylcytosine in only one sister chromatid)—a typical sign of chorionic cytotrophoblast cells. Our results suggest that the phenomena of interchromatid TL asymmetry and its association to 5hmC patterns in chorionic cytotrophoblast, which are potentially linked to telomere lengthening through recombination, are inherent to the development programme. The TL differences in chorionic cytotrophoblast that are associated with karyotype and embryo viability seem to be determined by heredity rather than telomere elongation mechanisms. The inheritance of long telomeres by a karyotypically abnormal embryo promotes his development, whereas TL in karyotypically normal first-trimester embryos does not seem to have a considerable impact on developmental capacity.
Databáze: OpenAIRE
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