Programmed Cell Death of Adult-Generated Hippocampal Neurons Is Mediated by the Proapoptotic Gene Bax
| Autor: | Woong Sun, Ronald W. Oppenheim, Hyun Kim, Ok Hee Park, Adam K. Winseck, Sharon Vinsant |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Aging
Programmed cell death Development/Plasticity/Repair Apoptosis Hippocampal formation Hippocampus Mice Cell Movement In Situ Nick-End Labeling medicine Animals Cell Proliferation bcl-2-Associated X Protein Mice Knockout Neurons biology General Neuroscience Dentate gyrus Neurogenesis Cell Differentiation Neural stem cell Doublecortin Cell biology Mice Inbred C57BL medicine.anatomical_structure Bromodeoxyuridine Proto-Oncogene Proteins c-bcl-2 nervous system Dentate Gyrus biology.protein Neuron Neuroscience |
| Zdroj: | The Journal of Neuroscience. 24:11205-11213 |
| ISSN: | 1529-2401 0270-6474 |
| DOI: | 10.1523/jneurosci.1436-04.2004 |
| Popis: | In the dentate gyrus (DG) of the adult mouse hippocampus, a substantial number of new cells are generated daily, but only a subset of these survive and differentiate into mature neurons, whereas the majority undergo programmed cell death (PCD). However, neither the intracellular machinery required for adult stem cell-derived neuronal death nor the biological implications of the significant loss of these newly generated cells have been examined. Several markers for apoptosis failed to reveal cell death in Bax-deficient mice, and this, together with a progressive increase in neuron number in the DG of the Bax knock-out, indicates that Bax is critical for the PCD of adult-generated hippocampal neurons. Whereas the proliferation of neural progenitor cells was not altered in the Bax-knock-out, there was an accumulation of doublecortin, calretinin+, and neuronal-specific nuclear protein+postmitotic neurons, suggesting that Bax-mediated PCD of adult-generated neurons takes place during an early phase of differentiation. The absence of PCD in the adult also influenced the migration and maturation of adult-generated DG neurons. These results suggest that PCD in the adult brain plays a significant role in the regulation of multiple aspects of adult neurogenesis. |
| Databáze: | OpenAIRE |
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